FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7147
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG287440.21267664754443No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC184225.773051630054567No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAA94413.208339163285295No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTC1672.3366447460472926No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC570.7975374282915908No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG350.4897159647404506No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG310.43374842591297047No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG240.33580523296488035No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG230.3218133482580104No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTCT220.3078214635511403No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG210.2938295788442703No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCTCACAGAGACCAAG180.2518539247236603No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAAG180.2518539247236603No Hit
CCTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG170.23786204001679023No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCTCACAGAGACCAAG160.22387015530992024No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCTCACAGAGACCAA150.20987827060305023No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG140.1958863858961802No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG140.1958863858961802No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG140.1958863858961802No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG130.1818945011893102No Hit
CCATTGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG120.16790261648244018No Hit
CCATTAGTGAGCTGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG110.15391073177557016No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG100.13991884706870014No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCTCACAGAGACCAAG100.13991884706870014No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCTCACAGAGACCAA100.13991884706870014No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG100.13991884706870014No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG90.12592696236183015No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAGGCATATCCAG80.11193507765496012No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.11193507765496012No Hit
AGATCATGTACGTCGGGGACGCCCGCAGCGTCACACAGAAGCATATCCAG80.11193507765496012No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG80.11193507765496012No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG80.11193507765496012No Hit
CCATTAGTGAGCTGGCCACCCTGTGATGATTGATGCTCACAGAGACCAAG80.11193507765496012No Hit
CCATTAGTGAGCTGGCCATACCAAGGTTGCTGGCTATACCTCGAGAAATC80.11193507765496012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)