FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5771
Total Bases835.3 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG191933.25246924276556No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC169129.301680817882513No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAA80213.897071564720154No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTC1933.344307745624675No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG500.8664009703690868No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG400.6931207762952695No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC390.6757927568878878No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG370.6411367180731242No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTCT220.38121642696239816No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCTCACAGAGACCAA200.34656038814763473No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG180.31190434933287126No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCTCACAGAGACCAA160.2772483105181078No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAAG160.2772483105181078No Hit
CCTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG150.25992029111072606No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG140.2425922717033443No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCTCACAGAGACCAAG140.2425922717033443No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG130.22526425229596256No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCTCACAGAGACCAAG130.22526425229596256No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG120.20793623288858082No Hit
CCTTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAAG110.19060821348119908No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCCCGAGAAATC110.19060821348119908No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG100.17328019407381737No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG100.17328019407381737No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG100.17328019407381737No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATATCTCGAGAAATC100.17328019407381737No Hit
AGATCATGTACGTCGGAGGATAAAGGCAACGTTCACAGAGACCAAGTCTC100.17328019407381737No Hit
CCATTAGTGGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.15595217466643563No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAGGCATATCCAG90.15595217466643563No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.15595217466643563No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACGGAAGCATATCCAG90.15595217466643563No Hit
CCATTAGTGAGCTGGCCATACCAAGGTTGCTGGCTATACCTCGAGAAATC90.15595217466643563No Hit
CCATTAGTGAGCTGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.1386241552590539No Hit
CCATTAGTAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.1386241552590539No Hit
TCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC80.1386241552590539No Hit
AGTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG70.12129613585167215No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGTTGGCTATACCTCGAGAAATC70.12129613585167215No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCTCACAGAGACCAA70.12129613585167215No Hit
CCATTAGTGAGCGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.12129613585167215No Hit
CCATTAGTGAGCTGGCCAACCCTGTGAAGATTGATGCTCACAGAGACCAA70.12129613585167215No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG70.12129613585167215No Hit
CCATAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.12129613585167215No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTACTGGCTATACCTCGAGAAATC70.12129613585167215No Hit
CCATTGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.12129613585167215No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCTCACAGAGACCAAG60.10396811644429041No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGATATC60.10396811644429041No Hit
CCATTAGTGAGCTGGCCAACCTGTGATGATTGATGCTCACAGAGACCAAG60.10396811644429041No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCTCACAGAGACCAAG60.10396811644429041No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG60.10396811644429041No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTCGCTGGCTATACCTCGAGAAATC60.10396811644429041No Hit
CCATTAGGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.10396811644429041No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGGGAAATC60.10396811644429041No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATACCCAG60.10396811644429041No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)