FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4820
Total Bases697.6 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG155032.15767634854772No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC132027.385892116182575No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAA82517.116182572614107No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTC1543.1950207468879666No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC330.6846473029045643No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG320.6639004149377593No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTCT310.6431535269709543No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG280.5809128630705395No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCTCACAGAGACCAAG220.4564315352697096No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG190.3941908713692946No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG180.37344398340248963No Hit
CCATTAGTGAGCTGGCCAACCCCGAGAACCAGTTCAGAGTGGACTACATC170.35269709543568467No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCTCACAGAGACCAA170.35269709543568467No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG140.29045643153526973No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCTCACAGAGACCAA130.2697095435684647No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG130.2697095435684647No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCTCACAGAGACCAAG120.24896265560165973No Hit
CCATTAGTGAGCTGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG120.24896265560165973No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAAG120.24896265560165973No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCTCACAGAGACCAA110.2282157676348548No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG100.2074688796680498No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.18672199170124482No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.16597510373443983No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCAGATCCAG80.16597510373443983No Hit
CCTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.14522821576763487No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACGGAAGCATATCCAG70.14522821576763487No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG70.14522821576763487No Hit
CCATAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.14522821576763487No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG70.14522821576763487No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATATTGATGCTCACAGAGACCAAG60.12448132780082986No Hit
CCATTAGTGAGCTGGCCAACCCTGGATGATTGATGCTCACAGAGACCAAG60.12448132780082986No Hit
CCATTAGTGAGCTGGCCAACCCTGTGAAGATTGATGCTCACAGAGACCAA60.12448132780082986No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG60.12448132780082986No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCGG60.12448132780082986No Hit
AGATCATGTACGTCGGGGGGATAAAGGCAACGTTCACAGAGACCAAGTCT60.12448132780082986No Hit
CCATTAGTGAGCTGGCCACCCTGTGATGATTGATGCTCACAGAGACCAAG60.12448132780082986No Hit
CCTTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC60.12448132780082986No Hit
AGATCATGTACGTCGGGGACGTCCGTAGCGTCACACAGAAGCATATCCAG60.12448132780082986No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGACTATACCTCGAGAAATC50.1037344398340249No Hit
AGTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.1037344398340249No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.1037344398340249No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCTCACAGAGACCAAG50.1037344398340249No Hit
CCATTAGTGAGCTGGCCAACCCGTGATGATTGATGCTCACAGAGACCAAG50.1037344398340249No Hit
CCATTAGTGAGTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG50.1037344398340249No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG50.1037344398340249No Hit
CCATTAGTGAGCGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG50.1037344398340249No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCTCACAGAGACCAAG50.1037344398340249No Hit
CCTTTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAA50.1037344398340249No Hit
AGATCATGTACGTCGGGGATAAAGGCAACGTTCACAGAGACCAAGTCTCT50.1037344398340249No Hit
CCATTGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG50.1037344398340249No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCCCGAGAAATC50.1037344398340249No Hit
CCATTAGTGAGCTGCCAACCCTGTGATGATTGATGCTCACAGAGACCAAG50.1037344398340249No Hit
AGATCATGTACGTCGGGGGATAAAGGCAACGTTCACAGAGACCAAGTCTC50.1037344398340249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)