FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4048
Total Bases585.8 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG142835.276679841897234No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC120929.866600790513836No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAA50512.475296442687748No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTC1032.544466403162055No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG320.7905138339920948No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC270.66699604743083No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCTCACAGAGACCAA200.4940711462450593No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG180.4446640316205534No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTCT170.4199604743083004No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG120.2964426877470355No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCTCACAGAGACCAAG110.2717391304347826No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCTCACAGAGACCAAG100.24703557312252966No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG90.2223320158102767No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGATATC90.2223320158102767No Hit
CCATTAGTGAGCTGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.2223320158102767No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.2223320158102767No Hit
CCTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.1976284584980237No Hit
CCATAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.1976284584980237No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.1976284584980237No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.1976284584980237No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCCCGAGAAATC80.1976284584980237No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG80.1976284584980237No Hit
AGATCATGTACGTGGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG70.17292490118577075No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAAG70.17292490118577075No Hit
CCATTATGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.14822134387351776No Hit
CCATTAGTGAGCTGGCCAACCCCGAGAACCAGTTCAGAGTGGACTACATT60.14822134387351776No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG60.14822134387351776No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG60.14822134387351776No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG60.14822134387351776No Hit
AGTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG60.14822134387351776No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG60.14822134387351776No Hit
CCATTAGTAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.14822134387351776No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAACATATCCAG50.12351778656126483No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAGGCATATCCAG50.12351778656126483No Hit
CCTTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAAG50.12351778656126483No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTTACACAGAAGCATATCCAG50.12351778656126483No Hit
CCATTAGTGAGCTGGCCAACCTGTGATGATTGATGCTCACAGAGACCAAG50.12351778656126483No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCTCACAGAGACCAA50.12351778656126483No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCGTATCCAG50.12351778656126483No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG50.12351778656126483No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)