FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4963
Total Bases716.2 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT176735.60346564577876No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA93618.859560749546645No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT3216.467862180132984No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT2745.520854321982672No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA2054.130566189804553No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTCCATGTTGTAGAACCAT280.5641748942172073No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG240.48357848075760623No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGCCATGTTGTAGAAC210.4231311706629055No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGCCATGTTGTAGAAC170.34253475720330445No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC170.34253475720330445No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGCCATGTTGTAGAA170.34253475720330445No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG160.3223856538384042No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT120.24178924037880312No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGCCATGTTGTAGAAC110.2216401370139029No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT110.2216401370139029No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGCCATGTTGTAGAA100.20149103364900264No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.20149103364900264No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.20149103364900264No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT100.20149103364900264No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC100.20149103364900264No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC90.18134193028410237No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG80.1611928269192021No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG80.1611928269192021No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT80.1611928269192021No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG80.1611928269192021No Hit
ACGTAGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT80.1611928269192021No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT70.14104372355430184No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCACTCGATCTGGT70.14104372355430184No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGTGCCCATTCGATCTGGT70.14104372355430184No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGCCATGTTGTAGAAC70.14104372355430184No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT70.14104372355430184No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG60.12089462018940156No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAGCTGGT60.12089462018940156No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCCTAATGGCCATGTTGTAGAAC60.12089462018940156No Hit
GCATCAATCATCACAGGGTTGGCCGCTCACTAATGGCCATGTTGTAGAAC60.12089462018940156No Hit
ACGTTGCCTTTATCCCCCCGACGTACATGATCTCCATGTTGTAGAACCAT60.12089462018940156No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT60.12089462018940156No Hit
ACGTTGCCTTAATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.12089462018940156No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGACCTGGT60.12089462018940156No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCACATTCGATCTGGT60.12089462018940156No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGTT50.10074551682450132No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGGGTGGGGCCCATTCGATCTGGT50.10074551682450132No Hit
ACGTTGCCTTTATCCTCCGACGTACATGATCTCCATGTTGTAGAACCATG50.10074551682450132No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGAGGGGCCCATTCGATCTGGT50.10074551682450132No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGCCATGTTGTAGAAC50.10074551682450132No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC50.10074551682450132No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.10074551682450132No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC50.10074551682450132No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG50.10074551682450132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)