FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5774
Total Bases829.6 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT202034.98441288534811No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT92716.054728091444407No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA64411.153446484239696No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT3105.368895046761344No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA1532.649809490820921No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTCCATGTTGTAGAACCAT350.6061655697956356No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG250.4329754069968826No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT240.4156563907170073No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGCCATGTTGTAGAA180.31174229303775547No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT170.29442327675788016No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG160.27710426047800485No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGCCATGTTGTAGAAC150.25978524419812954No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT150.25978524419812954No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG140.24246622791825423No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGCCATGTTGTAGAAC120.20782819535850364No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG120.20782819535850364No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT120.20782819535850364No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGCCATGTTGTAGAAC110.19050917907862833No Hit
ACGTAGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT110.19050917907862833No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG110.19050917907862833No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGCCATGTTGTAGAA100.17319016279875304No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.17319016279875304No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG100.17319016279875304No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC90.15587114651887773No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT90.15587114651887773No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC90.15587114651887773No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.15587114651887773No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG70.12123311395912711No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCGTTCGATCTGGT70.12123311395912711No Hit
ACGTTGCCTTTATCCTCCGACGTACATGATCTCCATGTTGTAGAACCATG70.12123311395912711No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC70.12123311395912711No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.12123311395912711No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGCGGGGCCCATTCGATCTGGT70.12123311395912711No Hit
ACGTTGCCTTTATCCTCCCAGATCCGGGAGTGGGGCCCATTCGATCTGGT70.12123311395912711No Hit
ACGTTGCCTTTATCCCCGACGTACATGATCTCCATGTTGTAGAACCATGT70.12123311395912711No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGCCCATTCGATCTGGTG70.12123311395912711No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT70.12123311395912711No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG70.12123311395912711No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.10391409767925182No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGCTGTCCCTCTT60.10391409767925182No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGAGGGGCCCATTCGATCTGGT60.10391409767925182No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT60.10391409767925182No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC60.10391409767925182No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT60.10391409767925182No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)