FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7046
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT212430.1447629860914No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT188326.724382628441667No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA80511.424921941527108No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA1702.4127164348566565No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTCCATGTTGTAGAACCAT310.43996593812091966No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT270.38319613965370425No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG250.35481124042009654No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT200.2838489923360772No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGCCATGTTGTAGAAC200.2838489923360772No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG180.2554640931024695No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGCCATGTTGTAGAA170.24127164348566565No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG150.21288674425205792No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGCCATGTTGTAGAAC140.19869429463525404No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG140.19869429463525404No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT140.19869429463525404No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGCCATGTTGTAGAAC130.18450184501845018No Hit
GCATCAATCATCATGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG130.18450184501845018No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG130.18450184501845018No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG130.18450184501845018No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG120.17030939540164633No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.15611694578484245No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGCCATGTTGTAGAA110.15611694578484245No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.15611694578484245No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT110.15611694578484245No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.15611694578484245No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGCTGTCCCTCTT100.1419244961680386No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT100.1419244961680386No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.1419244961680386No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.1419244961680386No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.1419244961680386No Hit
ACGTTGCCTTTATCCTCCCACAGAGCCTTGGCATGCTCATAGAATTCGGG90.12773204655123474No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG90.12773204655123474No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.12773204655123474No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT90.12773204655123474No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT80.11353959693443089No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGACGTCCCTCTT80.11353959693443089No Hit
CGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCAT80.11353959693443089No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGCCATGTTGTAGAAC80.11353959693443089No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGCCATGTTGTAGAAC80.11353959693443089No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)