FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7375
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT206528.000000000000004No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT146619.877966101694916No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA83611.335593220338982No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT6578.908474576271185No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA1401.8983050847457625No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTCCATGTTGTAGAACCAT330.44745762711864406No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT260.3525423728813559No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG250.3389830508474576No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG170.2305084745762712No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGCCATGTTGTAGAAC160.21694915254237288No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGCCATGTTGTAGAAC140.18983050847457628No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGCCATGTTGTAGAA140.18983050847457628No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT140.18983050847457628No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG130.17627118644067796No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCACTCTT130.17627118644067796No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC130.17627118644067796No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT130.17627118644067796No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG130.17627118644067796No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGCCATGTTGTAGAA120.16271186440677965No Hit
ACGTTGCCTTTATCCCCCCGACGTACATGATCTCCATGTTGTAGAACCAT120.16271186440677965No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT120.16271186440677965No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT110.14915254237288136No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.14915254237288136No Hit
GCATCAATCTTCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT110.14915254237288136No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG110.14915254237288136No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG110.14915254237288136No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC100.13559322033898305No Hit
ACGTTGCCTTTAACCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT100.13559322033898305No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.13559322033898305No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT100.13559322033898305No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.13559322033898305No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT100.13559322033898305No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG90.12203389830508474No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGCCCCTCTT90.12203389830508474No Hit
GCATCAATCATCACAGGGTTGGGCTCGAGAAATCACGAGATGTCCCTCTT90.12203389830508474No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAGTCACGAGATGTCCCTCTT90.12203389830508474No Hit
ACGTTGCCTTTATCCTCCCAGATCCGGGAGTGGGGCCCATTCGATCTGGT90.12203389830508474No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGGTGTCCCTCTT90.12203389830508474No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG90.12203389830508474No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG90.12203389830508474No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCTATCTGGT80.10847457627118644No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG80.10847457627118644No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG80.10847457627118644No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG80.10847457627118644No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC80.10847457627118644No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC80.10847457627118644No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCAT80.10847457627118644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)