FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4967
Total Bases714.7 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT192738.79605395611033No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA70114.113146768673243No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT69614.012482383732635No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA1122.2548822226696195No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT240.4831890477149185No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTCCATGTTGTAGAACCAT210.4227904167505537No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG210.4227904167505537No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG170.34225890879806725No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGCCATGTTGTAGAA150.30199315482182404No Hit
ACGTTGCCTTTATCCTCCCACAGAGCCTTGGCATGCTCATAGAATTCGGG140.28186027783370243No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGCCATGTTGTAGAAC130.2617274008455808No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT130.2617274008455808No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG120.24159452385745925No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGCCATGTTGTAGAAC110.22146164686933761No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG100.20132876988121604No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT100.20132876988121604No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGCCATGTTGTAGAA100.20132876988121604No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.18119589289309443No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.18119589289309443No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.18119589289309443No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC90.18119589289309443No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT90.18119589289309443No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT90.18119589289309443No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT90.18119589289309443No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG90.18119589289309443No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG80.16106301590497282No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTC80.16106301590497282No Hit
GCATCAATCATCACAGGGTTGGTGGCAGCTGGCAGTGGTTGGTGAATGTC80.16106301590497282No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT70.14093013891685122No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT70.14093013891685122No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG70.14093013891685122No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGCGGGGCCCATTCGATCTGGT70.14093013891685122No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT70.14093013891685122No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGC70.14093013891685122No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC70.14093013891685122No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT70.14093013891685122No Hit
ACGTTGCCATTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.12079726192872962No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC60.12079726192872962No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC60.12079726192872962No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATACGATCTGGT60.12079726192872962No Hit
ACGTTGCCTTTATCCTCCGACGTACATGATCTCCATGTTGTAGAACCATG50.10066438494060802No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGCCATGTTGTAGAAC50.10066438494060802No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC50.10066438494060802No Hit
ACGTTGCCTTTATCCTCCCAGATCCTGGAGTGGGGCCCATTCGATCTGGT50.10066438494060802No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGACCATGTTGTAGAA50.10066438494060802No Hit
ACGTTCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.10066438494060802No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT50.10066438494060802No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGTGCCCATTCGATCTGGT50.10066438494060802No Hit
GCATCAATCTTCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA50.10066438494060802No Hit
ACGTTGCCTTTATCCTCCCAGATCCGGGAGTGGGGCCCATTCGATCTGGT50.10066438494060802No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGA50.10066438494060802No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGG50.10066438494060802No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGAGCCCATTCGATCTGGT50.10066438494060802No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG50.10066438494060802No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGACCTGGT50.10066438494060802No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTAGATCTGGT50.10066438494060802No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)