FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8993
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGA215223.929723117980654No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT155317.268986989881018No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA114512.732124986100299No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT108012.009340598243078No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT4735.259646391637941No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA1381.5345268542199488No Hit
GCATCAATCATCAAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGAC360.40031135327476924No Hit
GCATCAATCATACAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGAC230.2557544757033248No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGCCATGTTGTAGAA220.24463471589013674No Hit
GCATCATCATCACAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGAC200.2223951962637607No Hit
GCATCAATCATCACGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGAC180.20015567663738462No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTCCATGTTGTAGAACCAT180.20015567663738462No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG180.20015567663738462No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGCCATGTTGTAGAAC160.17791615701100857No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG160.17791615701100857No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC160.17791615701100857No Hit
GCATCAATCTTCACAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGA160.17791615701100857No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGCCATGTTGTAGAA140.1556766373846325No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG140.1556766373846325No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGCCATGTTGTAGAAC130.14455687757144448No Hit
GATCAATCATCACAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGAC130.14455687757144448No Hit
GCATCAATCATCCAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGAC120.13343711775825642No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC120.13343711775825642No Hit
GCATCAATCATCACAGGGTTGGACGTAGGGGCCCTTGCGAGCAGGGTTGA110.12231735794506837No Hit
GCATCAATATCACAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGAC110.12231735794506837No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGCCATGTTGTAGAAC100.11119759813188035No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGCCATGTTGTAGAAC90.10007783831869231No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT90.10007783831869231No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT90.10007783831869231No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG90.10007783831869231No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC90.10007783831869231No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT90.10007783831869231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)