FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6364
Total Bases921.2 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG240437.774984286612195No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC159725.094280326838465No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAA90414.2049025769956No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTC1913.001257071024513No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC450.7071024512884978No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG400.6285355122564426No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG340.5342551854179761No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTCT290.4556882463859208No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG280.43997485857950974No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCTCACAGAGACCAA230.36140791954745444No Hit
CCATTAGTGAGCTGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG220.34569453174104336No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG170.26712759270898806No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCTCACAGAGACCAA160.251414204902577No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG150.23570081709616592No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG150.23570081709616592No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG140.21998742928975487No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG120.18856065367693275No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG120.18856065367693275No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG120.18856065367693275No Hit
CCATTAGTGAGCTGGCCACACCAAGGTTGCTGGCTATACCTCGAGAAATC120.18856065367693275No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCTCACAGAGACCAAG100.15713387806411064No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCTCACAGAGACCAAG100.15713387806411064No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG100.15713387806411064No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG100.15713387806411064No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG100.15713387806411064No Hit
AGATCATGTCGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.14142049025769957No Hit
CCTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.14142049025769957No Hit
AGATCATGTACGTGGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG90.14142049025769957No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCTCACAGAGACCAAG90.14142049025769957No Hit
CCATTAGTGAGCGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.14142049025769957No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG90.14142049025769957No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAAG90.14142049025769957No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG80.1257071024512885No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAGGCATATCCAG80.1257071024512885No Hit
AGATCATGTACGTCGGGGACGCCCGCAGCGTCACACAGAAGCATATCCAG80.1257071024512885No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCTCACAGAGACCAAG80.1257071024512885No Hit
CCATAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.1257071024512885No Hit
AGATCATGTACGTCGGGGACGTCTGCAGCGTCACACAGAAGCATATCCAG80.1257071024512885No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.1257071024512885No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG80.1257071024512885No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG70.10999371464487744No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCTCACAGAGACCAA70.10999371464487744No Hit
CCATTAGTGAGCTGGCCAAGCCAAGGTTGCTGGCTATACCTCGAGAAATC70.10999371464487744No Hit
CCATTAGTGAGCTGGCCAACCCTGTGAAGATTGATGCTCACAGAGACCAA70.10999371464487744No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCGGGCTATACCTCGAGAAATC70.10999371464487744No Hit
AGATCATGTACGTCGGAGGATAAAGGCAACGTTCACAGAGACCAAGTCTC70.10999371464487744No Hit
CCATTAGTAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.10999371464487744No Hit
CCATTAGTGAGCTGGCCACCCTGTGATGATTGATGCTCACAGAGACCAAG70.10999371464487744No Hit
CCATTAGTGAGCTGGCCATACCAAGGTTGCTGGCTATACCTCGAGAAATC70.10999371464487744No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)