FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6364
Total Bases913.4 kbp
Sequences flagged as poor quality0
Sequence length135-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT203832.02388434946574No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT145522.862979258328096No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA79812.539283469516027No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA1772.781269641734758No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTCCATGTTGTAGAACCAT280.43997485857950974No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAATGGGGCCCATTCGATCTGGT230.36140791954745444No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGCCATGTTGTAGAA210.3299811439346323No Hit
GCATCAATCTTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT200.3142677561282213No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG190.29855436832181015No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT180.28284098051539913No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG170.26712759270898806No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT130.2042740414833438No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGCCATGTTGTAGAA130.2042740414833438No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG130.2042740414833438No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG120.18856065367693275No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG120.18856065367693275No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG120.18856065367693275No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.17284726587052168No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGCTGTCCCTCTT100.15713387806411064No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAGTCGCGAGATGTCCCTCTT100.15713387806411064No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.15713387806411064No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG100.15713387806411064No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTC100.15713387806411064No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGCCATGTTGTAGAAC90.14142049025769957No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC90.14142049025769957No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGCCCCTCTT90.14142049025769957No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT90.14142049025769957No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGCCATGTTGTAGAAC80.1257071024512885No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT80.1257071024512885No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT80.1257071024512885No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGCCATGTTGTAGAAC80.1257071024512885No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT80.1257071024512885No Hit
GCATCAATCATCACAGGGTTGGACTCGGGAAATCGCGAGATGTCCCTCTT70.10999371464487744No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGGGATGTCCCTCTT70.10999371464487744No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCAT70.10999371464487744No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC70.10999371464487744No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTGTT70.10999371464487744No Hit
GCATCAATCATCACCGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT70.10999371464487744No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTA70.10999371464487744No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC70.10999371464487744No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.10999371464487744No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)