FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5261
Total Bases760.5 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC257448.92605968447064No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAA87016.53678007983273No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG59711.347652537540391No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTC1562.9652157384527653No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC741.4065766964455428No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG551.045428625736552No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG430.8173351073940316No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTCT380.7222961414179814No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG340.6462649686371412No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCTCACAGAGACCAAG300.5702337958563011No Hit
CCTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG200.38015586390420075No Hit
CCATTAGTGAGCTGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG170.3231324843185706No Hit
CCATTAGTAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG160.3041246911233606No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCTCACAGAGACCAA150.28511689792815054No Hit
CCTTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC150.28511689792815054No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCTCACAGAGACCAA140.2661091047329405No Hit
CCATTGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG130.24710131153773046No Hit
CCATTAGTGGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG120.22809351834252042No Hit
CCATTAGTGAGCGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG120.22809351834252042No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAAG120.22809351834252042No Hit
CCATTATGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG100.19007793195210038No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCTCACAGAGACCAAG100.19007793195210038No Hit
CCATTAGTGGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAAG100.19007793195210038No Hit
CCATTAGTGAGCTGGCCACACCAAGGTTGCTGGCTATACCTCGAGAAATC90.1710701387568903No Hit
CCATTAGTGAGCTGGCCAAACCAGGGTTGCTGGCTATACCTCGAGAAATC80.1520623455616803No Hit
CCATAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.1520623455616803No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCTCACAGAGACCAAG70.13305455236647026No Hit
CCATTAGTGAGCTGGCCACCCTGTGATGATTGATGCTCACAGAGACCAAG70.13305455236647026No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAACC70.13305455236647026No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCTCACAGAGACCAA70.13305455236647026No Hit
CAATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC70.13305455236647026No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATATCTCGAGAAATC60.11404675917126021No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCTCACAGAGACCAAG60.11404675917126021No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG60.11404675917126021No Hit
CCATTAGGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.11404675917126021No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCTCACAGAGACCAAG60.11404675917126021No Hit
CCATTAGTGAGCTGGCCAAACCAAGGCTGCTGGCTATACCTCGAGAAATC60.11404675917126021No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGGGAAATC60.11404675917126021No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG60.11404675917126021No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)