FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6047
Total Bases868.6 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT176629.204564246733916No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT151725.08681991069952No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA73412.13825037208533No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA1853.0593682817926244No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTCCATGTTGTAGAACCAT340.5622622788159418No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG290.47957664957830326No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGCCATGTTGTAGAAC190.3142053911030263No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT190.3142053911030263No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT160.2645940135604432No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGCCATGTTGTAGAA160.2645940135604432No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG150.2480568877129155No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT130.2149826360178601No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGCTGTCCCTCTT130.2149826360178601No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG130.2149826360178601No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC120.1984455101703324No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG120.1984455101703324No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG120.1984455101703324No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGCCATGTTGTAGAA110.1819083843228047No Hit
ACGTTGCCTTTATCCTCCCACAGAGCCTTGGCATGCTCATAGAATTCGGG110.1819083843228047No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.1819083843228047No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGCCATGTTGTAGAAC110.1819083843228047No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG110.1819083843228047No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC110.1819083843228047No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAACTCGCGAGATGTCCCTCTT100.165371258475277No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.165371258475277No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG100.165371258475277No Hit
GCATCAATCTTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT100.165371258475277No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.14883413262774928No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT90.14883413262774928No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGCCATGTTGTAGAAC80.1322970067802216No Hit
ACGTTGCCTTTATCCTCCCAGATCCTGGAGTGGGGCCCATTCGATCTGGT80.1322970067802216No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT80.1322970067802216No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT80.1322970067802216No Hit
ACGTTGCCTTTATCCTCCCAGATCCGGGAGTGGGGCCCATTCGATCTGGT80.1322970067802216No Hit
GCATCAATCATCACCGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT80.1322970067802216No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT80.1322970067802216No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG80.1322970067802216No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG80.1322970067802216No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT70.11575988093269389No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG70.11575988093269389No Hit
ACGTTGCCTTTATCCTCCCAGATCAAGGAGTGGGGCCCATTCGATCTGGT70.11575988093269389No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGCCATGTTGTAGAAC70.11575988093269389No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)