FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6159
Total Bases884.6 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT205333.33333333333333No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT93115.116090274395194No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA71111.544081831466146No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT5589.059912323429128No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA1562.5328787140769604No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG200.324728040266277No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT190.3084916382529631No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTCCATGTTGTAGAACCAT170.27601883422633544No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGCCATGTTGTAGAAC160.2597824322130216No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT150.24354603019970775No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGCCATGTTGTAGAAC140.22730962818639389No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT120.1948368241597662No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT120.1948368241597662No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT120.1948368241597662No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG110.17860042214645236No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT110.17860042214645236No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT100.1623640201331385No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG100.1623640201331385No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT100.1623640201331385No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.1623640201331385No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT100.1623640201331385No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG100.1623640201331385No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC90.14612761811982464No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT90.14612761811982464No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGCCATGTTGTAGAA90.14612761811982464No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG80.1298912161065108No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG80.1298912161065108No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG80.1298912161065108No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG80.1298912161065108No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCACTCTT70.11365481409319694No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG70.11365481409319694No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.11365481409319694No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGACCTGGT70.11365481409319694No Hit
ACGTAGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT70.11365481409319694No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)