FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4041
Total Bases581.5 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT199649.393714427122No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA46111.408067310071765No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT2666.582529076961148No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA872.1529324424647367No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT310.7671368473150211No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT200.4949269982677555No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG190.47018064835436774No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG170.4206879485275922No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTCCATGTTGTAGAACCAT160.3959415986142044No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG150.3711952487008166No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG150.3711952487008166No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGCCATGTTGTAGAAC140.34644889878742885No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT140.34644889878742885No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT120.2969561989606533No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT110.27220984904726553No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG110.27220984904726553No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.24746349913387775No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT100.24746349913387775No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.24746349913387775No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT90.22271714922048996No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCTATCTGGT80.1979707993071022No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT80.1979707993071022No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTAGGGCCCATTCGATCTGGT70.17322444939371442No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT70.17322444939371442No Hit
ACGTTGCCTTTATCCTCCCAGATCCGGGAGTGGGGCCCATTCGATCTGGT60.14847809948032664No Hit
ACGTTGCCTTTATCCTCCCAGATCCTGGAGTGGGGCCCATTCGATCTGGT60.14847809948032664No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGCCATGTTGTAGAAC60.14847809948032664No Hit
ACGTTCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.14847809948032664No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGTGCCCATTCGATCTGGT50.12373174956693887No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTAGT50.12373174956693887No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAGCTGGT50.12373174956693887No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATCCGATCTGGT50.12373174956693887No Hit
ACGTTGCCTTTATCCACCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.12373174956693887No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGCCATGTTGTAGAAC50.12373174956693887No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.12373174956693887No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG50.12373174956693887No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGTCCATTCGATCTGGT50.12373174956693887No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGA50.12373174956693887No Hit
ACGTTGCCTTAATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.12373174956693887No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTAGATCTGGT50.12373174956693887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)