FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5222
Total Bases750.5 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT221842.47414783607813No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT81915.683646112600535No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA4839.249329758713138No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA1062.029873611643049No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG290.5553427805438529No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT260.49789352738414405No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG230.4404442742244351No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTCCATGTTGTAGAACCAT180.3446955189582535No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT170.3255457679050172No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG170.3255457679050172No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT150.2872462657985446No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT140.2680965147453083No Hit
ACGTTGCCTTTATCCTCCCAGATCCGGGAGTGGGGCCCATTCGATCTGGT120.2297970126388357No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG110.21064726158559938No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG110.21064726158559938No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT110.21064726158559938No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT110.21064726158559938No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT100.19149751053236308No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT100.19149751053236308No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGCCATGTTGTAGAAC90.17234775947912676No Hit
ACGTTGCCTTTATCCTCCCACAGAGCCTTGGCATGCTCATAGAATTCGGG90.17234775947912676No Hit
ACGTTGCCTTTATCCTCCCAGACCCAGGAGTGGGGCCCATTCGATCTGGT90.17234775947912676No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC90.17234775947912676No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGCCATGTTGTAGAA90.17234775947912676No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT90.17234775947912676No Hit
ACGTAGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT90.17234775947912676No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCTATCTGGT80.15319800842589046No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG80.15319800842589046No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCAT80.15319800842589046No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAGCTGGT80.15319800842589046No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGCCATGTTGTAGAA70.13404825737265416No Hit
ACGTTGCCTTTATCCTCCCAGATCCTGGAGTGGGGCCCATTCGATCTGGT70.13404825737265416No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG70.13404825737265416No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT70.13404825737265416No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGACCTGGT70.13404825737265416No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.13404825737265416No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGGTCTGGT70.13404825737265416No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG70.13404825737265416No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT60.11489850631941785No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAATCGATCTGGT60.11489850631941785No Hit
ACGTTGCCTTTAACCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.11489850631941785No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGCCATGTTGTAGAAC60.11489850631941785No Hit
ACGTTGCCTTTTTCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.11489850631941785No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG60.11489850631941785No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTC60.11489850631941785No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT60.11489850631941785No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGAGCCCATTCGATCTGGT60.11489850631941785No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTTGT60.11489850631941785No Hit
GCATCAATCTTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT60.11489850631941785No Hit
ACGATGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.11489850631941785No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)