FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5872
Total Bases849.8 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG208035.42234332425068No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC183331.21594005449591No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAA65711.188692098092643No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTC1232.0946866485013627No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC530.9025885558583105No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG440.7493188010899182No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG330.5619891008174387No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG300.510899182561308No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTCT190.32356948228882837No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCTCACAGAGACCAA170.2895095367847411No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG160.2724795640326975No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCTCACAGAGACCAAG160.2724795640326975No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG150.255449591280654No Hit
CCATAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG140.23841961852861035No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG130.22138964577656678No Hit
CCATTAGTGAGCTGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG110.18732970027247955No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG110.18732970027247955No Hit
CCTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG100.17029972752043596No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG100.17029972752043596No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG100.17029972752043596No Hit
CCATTAGTGGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.15326975476839239No Hit
CCATTAGTGAGCGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.15326975476839239No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG90.15326975476839239No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCTCACAGAGACCAAG90.15326975476839239No Hit
AGATCATGTACGTGGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG80.13623978201634876No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.13623978201634876No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG80.13623978201634876No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTACTGGCTATACCTCGAGAAATC80.13623978201634876No Hit
AGATCATGACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.13623978201634876No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.13623978201634876No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACGGAAGCATATCCAG70.11920980926430517No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAGTC70.11920980926430517No Hit
CCATTAGTGAGCTGGCCACCCTGTGATGATTGATGCTCACAGAGACCAAG70.11920980926430517No Hit
CCATTGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.11920980926430517No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG70.11920980926430517No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG60.10217983651226158No Hit
CCATTAGCGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC60.10217983651226158No Hit
AGTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG60.10217983651226158No Hit
CCATTAGTGAGCTGGCCAACCCGTGATGATTGATGCTCACAGAGACCAAG60.10217983651226158No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGGAGCATATCCAG60.10217983651226158No Hit
AGATCATGTACGTCGGGGACGCCCGCAGCGTCACACAGAAGCATATCCAG60.10217983651226158No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCCGGCTATACCTCGAGAAATC60.10217983651226158No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCTCACAGAGACCAAG60.10217983651226158No Hit
CCATTAGTGAGCTGGCCAATCCAAGGTTGCTGGCTATACCTCGAGAAATC60.10217983651226158No Hit
CCATTAGTGAGCTGGCCAACCCTGTGAAGATTGATGCTCACAGAGACCAA60.10217983651226158No Hit
CCATTATGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.10217983651226158No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGGAATC60.10217983651226158No Hit
CCATTAGTAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.10217983651226158No Hit
CCATTAGGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.10217983651226158No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCTCACAGAGACCAA60.10217983651226158No Hit
CCATTAGTGAGCTGGCCACACCAAGGTTGCTGGCTATACCTCGAGAAATC60.10217983651226158No Hit
CCATTAGTGAGCTGGCCATACCAAGGTTGCTGGCTATACCTCGAGAAATC60.10217983651226158No Hit
AGATCATGTACGTCGGGGACGTCTGCAGCGTCACACAGAAGCATATCCAG60.10217983651226158No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAAG60.10217983651226158No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTGTACCTCGAGAAATC60.10217983651226158No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)