FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5565
Total Bases804.7 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC248244.60017969451931No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG140425.22911051212938No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAA4648.337825696316262No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC801.4375561545372868No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTC591.060197663971249No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG550.9883198562443846No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG450.8086253369272237No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG430.7726864330637916No Hit
CCTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG230.4132973944294699No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG170.3054806828391734No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAAG150.2695417789757413No Hit
CCATTAGTGGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG140.25157232704402516No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG140.25157232704402516No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG130.23360287511230907No Hit
CCATAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG120.215633423180593No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG120.215633423180593No Hit
CCATTAGTAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG110.1976639712488769No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCTCACAGAGACCAA100.17969451931716085No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTCT100.17969451931716085No Hit
CCATTAGTGAGCGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.16172506738544476No Hit
CCATTATGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.16172506738544476No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGGAATC90.16172506738544476No Hit
CCATTAGTGAGCTGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.16172506738544476No Hit
CCATTAGAGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC90.16172506738544476No Hit
CCATTAGTGAGCTGGCCACACCAAGGTTGCTGGCTATACCTCGAGAAATC90.16172506738544476No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTACACCTCGAGAAATC90.16172506738544476No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.14375561545372867No Hit
CCATTAGTGAGTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.14375561545372867No Hit
CCATTAGGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.14375561545372867No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCTCACAGAGACCAA80.14375561545372867No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG70.12578616352201258No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAACC70.12578616352201258No Hit
CCATTAGTGAGCTGGCCAAACCAAGGCTGCTGGCTATACCTCGAGAAATC70.12578616352201258No Hit
CCATTAGTGACTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.12578616352201258No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG70.12578616352201258No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGTTATACCTCGAGAAATC60.1078167115902965No Hit
CTATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC60.1078167115902965No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCTCACAGAGACCAAG60.1078167115902965No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCTCACAGAGACCAA60.1078167115902965No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG60.1078167115902965No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCTCACAGAGACCAAG60.1078167115902965No Hit
CCATTGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.1078167115902965No Hit
CCATTAGTGAGCTGGCCATACCAAGGTTGCTGGCTATACCTCGAGAAATC60.1078167115902965No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG60.1078167115902965No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)