FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9290
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT416444.82238966630786No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT158017.007534983853606No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA5305.705059203444564No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT4805.166846071044134No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA690.7427341227125942No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG580.6243272335844995No Hit
GCATCAATCTTCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT320.3444564047362756No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG290.31216361679224974No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG280.3013993541442411No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG250.2691065662002153No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG220.23681377825618943No Hit
GCATCAATCATCCCAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT220.23681377825618943No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG220.23681377825618943No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG210.22604951560818085No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT210.22604951560818085No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCACTCTT200.2152852529601722No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG190.20452099031216361No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG170.18299246501614638No Hit
ACGTTGCCTTTATCCTCCCACAGAGCCTTGGCATGCTCATAGAATTCGGG150.16146393972012918No Hit
GCATCAATCATCACCGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT150.16146393972012918No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCATGAGATGTCCCTCTT150.16146393972012918No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGCTGTCCCTCTT140.15069967707212056No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG140.15069967707212056No Hit
GCATCAATCATCACAGGGTTGGGCTCGAGAAATCACGAGATGTCCCTCTT140.15069967707212056No Hit
GCATCAATCATCATGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG130.13993541442411195No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGCCATGTTGTAGAAC120.12917115177610333No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTTG120.12917115177610333No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTC120.12917115177610333No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAACCACGAGATGTCCCTCTT120.12917115177610333No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG110.11840688912809472No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTA110.11840688912809472No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCACTT110.11840688912809472No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCCCTT110.11840688912809472No Hit
GCATCCATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT100.1076426264800861No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGGTGTCCCTCTT100.1076426264800861No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCAT100.1076426264800861No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)