FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3743
Total Bases541.4 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC146439.11301095378039No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG105828.266096713865885No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAA38010.152284263959391No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTC551.4694095645204381No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC431.1488111140796153No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG330.8816457387122629No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG300.8014961261020572No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG270.7213465134918514No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG150.4007480630510286No Hit
CCTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG140.37403152551429336No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG140.37403152551429336No Hit
CCATTAGTGAGCTGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG110.2938819129040876No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCTCACAGAGACCAAG100.26716537536735235No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAAG100.26716537536735235No Hit
CCATTAGTGAGCTGGCCAACCCCGAGAACCAGTTCAGAGTGGACTACATT90.24044883783061713No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCTCACAGAGACCAA90.24044883783061713No Hit
CCATTATGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.21373230029388193No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCTCACAGAGACCAA80.21373230029388193No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG70.18701576275714668No Hit
CCATTAGTGAGCTGGCCAACCCCGAGAACCAGTTCAGAGTGGACTACATC60.16029922522041143No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG60.16029922522041143No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGATATC60.16029922522041143No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG60.16029922522041143No Hit
CCTTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC60.16029922522041143No Hit
CCATTGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.16029922522041143No Hit
CCATTAGTGAGCTGGCCATACCAAGGTTGCTGGCTATACCTCGAGAAATC60.16029922522041143No Hit
CCATTAGTGAGCGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.16029922522041143No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCTCACAGAGACCAAG50.13358268768367618No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGTTATACCTCGAGAAATC50.13358268768367618No Hit
CCATTAGTGGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG50.13358268768367618No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACGGAAGCATATCCAG50.13358268768367618No Hit
AGTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.13358268768367618No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCGG50.13358268768367618No Hit
AGATCATGTACGTCGGGGATAAAGGCAACGTTCACAGAGACCAAGTCTCT50.13358268768367618No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGTTGGCTATACCTCGAGAAATC50.13358268768367618No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCTCACAGAGACCAAG50.13358268768367618No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCTCACAGAGACCAA50.13358268768367618No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATGCCTCGAGAAATC50.13358268768367618No Hit
AGATCAGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.13358268768367618No Hit
CCATTAGTGAGCTGGCCAAACCCGAGAACCAGTTCAGAGTGGACTACATT50.13358268768367618No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTCT50.13358268768367618No Hit
CCATTAGTGAGCTGGCCTTGTGGGGTCATGCTAAGTGCCATGTTTTGGAT40.10686615014694097No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG40.10686615014694097No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG40.10686615014694097No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCTCACAGAGACCAAG40.10686615014694097No Hit
CCTTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAAG40.10686615014694097No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTCGCTGGCTATACCTCGAGAAATC40.10686615014694097No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG40.10686615014694097No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTACTGGCTATACCTCGAGAAATC40.10686615014694097No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCTCACAGAGACCAAG40.10686615014694097No Hit
CCATTAGTGAGTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG40.10686615014694097No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCCCGAGAAATC40.10686615014694097No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG40.10686615014694097No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAGTC40.10686615014694097No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)