FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4942
Total Bases711.5 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT269254.47187373532982No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA4569.227033589639822No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT2194.43140428976123No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT1352.7316875758802106No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG430.8700930797248078No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA310.6272764063132336No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT230.4653986240388507No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG200.4046944556859571No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG180.36422501011736136No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT170.3439902873330636No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT150.3035208417644678No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT140.28328611898017No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG120.24281667341157423No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT120.24281667341157423No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGCCATGTTGTAGAAC110.22258195062727643No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGG110.22258195062727643No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT110.22258195062727643No Hit
ACGTTGCCTTTATCCTCCCACAGAGCCTTGGCATGCTCATAGAATTCGGG100.20234722784297854No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.20234722784297854No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT90.18211250505868068No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGTTCTGGT90.18211250505868068No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC90.18211250505868068No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGCCATGTTGTAGAAC80.16187778227438285No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT80.16187778227438285No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCGGGT80.16187778227438285No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGCCATGTTGTAGAA80.16187778227438285No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGC80.16187778227438285No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG80.16187778227438285No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGGGTGGGGCCCATTCGATCTGGT70.141643059490085No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT70.141643059490085No Hit
ACGTTGCCTTTATCCTCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.141643059490085No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGACCCATTCGATCTGGT70.141643059490085No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT70.141643059490085No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGA70.141643059490085No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTAGATCTGGT70.141643059490085No Hit
ACGTTGCCTTTATCCTCCCAGATCCCGGAGTGGGGCCCATTCGATCTGGT60.12140833670578711No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCTATCTGGT60.12140833670578711No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG60.12140833670578711No Hit
ACGTTGCCTTTTTCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.12140833670578711No Hit
ACGGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.12140833670578711No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGCCCATTCGATCTGGTG60.12140833670578711No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT60.12140833670578711No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGCCATGTTGTAGAA50.10117361392148927No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCACTCTT50.10117361392148927No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGAGGGGCCCATTCGATCTGGT50.10117361392148927No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCGCATTCGATCTGGT50.10117361392148927No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCACTCGATCTGGT50.10117361392148927No Hit
ACGTTGCCTTTATCCTCCCAGACCCAGGAGTGGGGCCCATTCGATCTGGT50.10117361392148927No Hit
ACGTTGCCTTTATCCTCCCAGATCAAGGAGTGGGGCCCATTCGATCTGGT50.10117361392148927No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGAGGCCCATTCGATCTGGT50.10117361392148927No Hit
ACGTTGCCTTTATCCTCCCAGATCCGGGAGTGGGGCCCATTCGATCTGGT50.10117361392148927No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGAGCCCATTCGATCTGGT50.10117361392148927No Hit
ACGTTGCCTTTATACTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.10117361392148927No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTCGT50.10117361392148927No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)