FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9008
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG518757.582149200710475No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTACCTCGAGAAATCGC145916.19671403197158No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAA6387.08259325044405No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTC640.7104795737122558No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTACCTCGAGAAATCGC480.5328596802841918No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG460.5106571936056838No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG460.5106571936056838No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG400.4440497335701598No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG350.3885435168738899No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG300.3330373001776199No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTACCTCGAGAAATCGCG280.3108348134991119No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTACCTCGAGAAATCGCG260.2886323268206039No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCGTATCCAG240.2664298401420959No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTACCTCGAGAAATCGCG230.2553285968028419No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG220.24422735346358795No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG220.24422735346358795No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG210.2331261101243339No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACGGAAGCATATCCAG210.2331261101243339No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG210.2331261101243339No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG210.2331261101243339No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG200.2220248667850799No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG160.17761989342806395No Hit
CCTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTACCTCGAGAAATCGCG150.16651865008880995No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAGGCATATCCAG140.15541740674955595No Hit
AGATCATGTACGTCGGGGCCGTCCGCAGCGTCACACAGAAGCATATCCAG140.15541740674955595No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG140.15541740674955595No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG140.15541740674955595No Hit
CCATTAGTGAGCTGGCCACACCAAGGTTGCTGGCTACCTCGAGAAATCGC130.14431616341030196No Hit
CCATTAGTGAGCTGGCCAACCCCGAGAACCAGTTCAGAGTGGACTACATC130.14431616341030196No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCGG130.14431616341030196No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCTCACAGAGACCAA130.14431616341030196No Hit
AGATCAGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG130.14431616341030196No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATACCCAG130.14431616341030196No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCACATCCAG120.13321492007104796No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAAG120.13321492007104796No Hit
AGATCATGTCGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG110.12211367673179398No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCTCACAGAGACCAAG110.12211367673179398No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG110.12211367673179398No Hit
AGTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG100.11101243339253995No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG100.11101243339253995No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCTCACAGAGACCAAG100.11101243339253995No Hit
CCATTAGTGGCTGGCCAAACCAAGGTTGCTGGCTACCTCGAGAAATCGCG100.11101243339253995No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)