FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8301
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG421650.78906155884834No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC222026.74376581134803No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAA4405.3005661968437545No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC700.8432718949524154No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG330.39754246476328153No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCA270.32526201662450305No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCA250.30116853391157694No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG240.28912179255511383No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG240.28912179255511383No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG220.26502830984218767No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCA210.2529815684857246No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG180.21684134441633537No Hit
CCATTATGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCA180.21684134441633537No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAA180.21684134441633537No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACGGAAGCATATCCAG170.2047946030598723No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG170.2047946030598723No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG160.19274786170340924No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG160.19274786170340924No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG160.19274786170340924No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG150.18070112034694613No Hit
AGATCATGTACGTCGGGGACGCCCGCAGCGTCACACAGAAGCATATCCAG150.18070112034694613No Hit
AGATCATGTACGTGGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG140.16865437899048308No Hit
AGATCATGTACGTCGGGGACATCCGCAGCGTCACACAGAAGCATATCCAG140.16865437899048308No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG140.16865437899048308No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG140.16865437899048308No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG140.16865437899048308No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTC140.16865437899048308No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG130.15660763763402No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG130.15660763763402No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG120.14456089627755692No Hit
CCTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCA120.14456089627755692No Hit
AATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG120.14456089627755692No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG110.13251415492109384No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCTCACAGAGACCAAG110.13251415492109384No Hit
CCATTAGTAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCA110.13251415492109384No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAGGCATATCCAG100.12046741356463078No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG100.12046741356463078No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCGG100.12046741356463078No Hit
AGATCATGTCGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.10842067220816769No Hit
GATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.10842067220816769No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCGTATCCAG90.10842067220816769No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)