FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8301
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT360343.40440910733647No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT200324.129622936995542No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA4044.866883508011083No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG390.46982291290205996No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT340.4095892061197446No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG300.36140224069389226No Hit
GCATCAATCTTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT300.36140224069389226No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG290.34935549933742926No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT240.28912179255511383No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGCTGTCCCTCTT230.2770750511986508No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG220.26502830984218767No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG220.26502830984218767No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGGTGTCCCTCTT210.2529815684857246No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT200.24093482712926156No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG190.22888808577279846No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG190.22888808577279846No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG180.21684134441633537No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG180.21684134441633537No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGCCCCTCTT170.2047946030598723No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA150.18070112034694613No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT140.16865437899048308No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT130.15660763763402No Hit
GCATCAATCATCACCGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT130.15660763763402No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGACGTCCCTCTT120.14456089627755692No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTA120.14456089627755692No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAACTCGCGAGATGTCCCTCTT110.13251415492109384No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT110.13251415492109384No Hit
GCATCAATCATCATGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.13251415492109384No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCCCTT110.13251415492109384No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG110.13251415492109384No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGGGATGTCCCTCTT100.12046741356463078No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCAT100.12046741356463078No Hit
GCATCAATCATCACAGGGTTGACTCGAGAAATCGCGAGATGTCCCTCTTG100.12046741356463078No Hit
GCATCAATCATCCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT100.12046741356463078No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTGTT100.12046741356463078No Hit
GCATCAATCATCACAGGGTTGGGCTCGAGAAATCGCGAGATGTCCCTCTT100.12046741356463078No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCTATCTGGT90.10842067220816769No Hit
GCATCAAACATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT90.10842067220816769No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG90.10842067220816769No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT90.10842067220816769No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.10842067220816769No Hit
GCATCAATCATCACAGGGTTGGACACGAGAAATCGCGAGATGTCCCTCTT90.10842067220816769No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)