FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301
Total Bases475.5 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT169551.34807634050288No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA58617.75219630415026No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA290.8785216601029991No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT190.5755831566192063No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG170.5149954559224478No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGCCATGTTGTAGAA150.45440775522568916No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG130.3938200545289306No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT130.3938200545289306No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT110.3332323538321721No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT110.3332323538321721No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCCGGT100.3029385034837928No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.3029385034837928No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT90.27264465313541353No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT80.24235080278703422No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT80.24235080278703422No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT80.24235080278703422No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.21205695243865494No Hit
ACGTTGCCTTTATCCTCCCAGATCCGGGAGTGGGGCCCATTCGATCTGGT70.21205695243865494No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGCCATGTTGTAGAAC70.21205695243865494No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGC70.21205695243865494No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCACTCGATCTGGT70.21205695243865494No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTCCATGTTGTAGAACCAT70.21205695243865494No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGACCTGGT70.21205695243865494No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.21205695243865494No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAAC60.18176310209027569No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAGCTGGT60.18176310209027569No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATCCGATCTGGT60.18176310209027569No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGGGTGGGGCCCATTCGATCTGGT60.18176310209027569No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT60.18176310209027569No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.18176310209027569No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGAGGGGCCCATTCGATCTGGT60.18176310209027569No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.18176310209027569No Hit
ACGTAGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.18176310209027569No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGTGCCCATTCGATCTGGT50.1514692517418964No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGCGGGGCCCATTCGATCTGGT50.1514692517418964No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAATCGATCTGGT50.1514692517418964No Hit
ACGTTGCCTTTATCCTCCCAGAACCAGGAGTGGGGCCCATTCGATCTGGT50.1514692517418964No Hit
GCATCAATAATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA50.1514692517418964No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.1514692517418964No Hit
ACGTTGCCTTAATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.1514692517418964No Hit
ACGTTGCCTTTATCCTCCCAGATCCCGGAGTGGGGCCCATTCGATCTGGT40.12117540139351711No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGCCATGTTGTAGAAC40.12117540139351711No Hit
ACGTTGCCTTTATCCTCCCAGATCAAGGAGTGGGGCCCATTCGATCTGGT40.12117540139351711No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGCCATGTTGTAGAAC40.12117540139351711No Hit
GCATCAATCTTCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA40.12117540139351711No Hit
ACGTTGCCTTTAACCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.12117540139351711No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT40.12117540139351711No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGCCATGTTGTAGAAC40.12117540139351711No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCTATTCGATCTGGT40.12117540139351711No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATACGATCTGGT40.12117540139351711No Hit
ACGTTGCCTTTTCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG40.12117540139351711No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTCGT40.12117540139351711No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)