FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6028
Total Bases871.8 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC241240.013271400132716No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG167327.753815527538155No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAA68711.396814863968148No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC711.1778367617783676No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTC420.696748506967485No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG410.6801592568015925No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG390.6469807564698076No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG350.5806237558062376No Hit
CCTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG170.28201725282017254No Hit
CCATTAGTGAGCTGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG170.28201725282017254No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG160.26542800265428No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCTCACAGAGACCAA160.26542800265428No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG150.24883875248838755No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCTCACAGAGACCAAG150.24883875248838755No Hit
CCATTAGTGGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG130.21566025215660253No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG130.21566025215660253No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG130.21566025215660253No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTCT120.19907100199071004No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCTCACAGAGACCAAG110.18248175182481752No Hit
CCATTAGTGAGCGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG110.18248175182481752No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG110.18248175182481752No Hit
CCTTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC110.18248175182481752No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCTCACAGAGACCAAG100.16589250165892502No Hit
CCATTATGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG100.16589250165892502No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG100.16589250165892502No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG90.14930325149303253No Hit
CCATTAGTGAGCTGGCCACACCAAGGTTGCTGGCTATACCTCGAGAAATC90.14930325149303253No Hit
CCATTAGTGAGCTGGCCATACCAAGGTTGCTGGCTATACCTCGAGAAATC90.14930325149303253No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAAG90.14930325149303253No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATATTGATGCTCACAGAGACCAAG80.13271400132714No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCACATCCAG80.13271400132714No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.13271400132714No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCCGGCTATACCTCGAGAAATC80.13271400132714No Hit
CCATTAGTAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.13271400132714No Hit
CCATAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.13271400132714No Hit
CCATTGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.13271400132714No Hit
CTATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC70.1161247511612475No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCTCACAGAGACCAA70.1161247511612475No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGGAATC70.1161247511612475No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTCGCTGGCTATACCTCGAGAAATC70.1161247511612475No Hit
CCATTAGGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.1161247511612475No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCTCACAGAGACCAA70.1161247511612475No Hit
CCATTAGAGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC70.1161247511612475No Hit
CCATTAGTGAGCTGGTCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC70.1161247511612475No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)