FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13844
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT521637.67697197341809No Hit
GCATCAATCATCACAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGA315022.753539439468362No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT12178.790811904073967No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA6344.579601271308871No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA650.4695174804969662No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG620.4478474429355677No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG460.33227390927477607No Hit
GCATCAATCATCAAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGAC420.30338052585957814No Hit
GCATCAATCTTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT390.2817104882981797No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG340.24559375902918232No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG340.24559375902918232No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGGTGTCCCTCTT300.21670037561398442No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG290.20947702976018492No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG280.20225368390638546No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGCTGTCCCTCTT270.19503033805258596No Hit
GCATCAATCTTCACAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGA270.19503033805258596No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG260.18780699219878647No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT250.180583646344987No Hit
GCATCAATCATACAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGAC240.17336030049118753No Hit
GCATCAATCATCACAGGGTTGGGCGTAGAGGCCCTTGCGAGCAGGGTTGA240.17336030049118753No Hit
GCATCAATATCACAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGAC230.16613695463738803No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG220.15891360878358857No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG220.15891360878358857No Hit
GCATCAATCATCACCGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT210.15169026292978907No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG210.15169026292978907No Hit
GCATCAATCATCACAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGG200.1444669170759896No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTA190.1372435712221901No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT180.13002022536839064No Hit
GCATAATCATCACAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGAC180.13002022536839064No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGCCCCTCTT180.13002022536839064No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGCCATGTTGTAGAAC170.12279687951459116No Hit
GCATCATCATCACAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGAC170.12279687951459116No Hit
GCATCAATCATCCAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGAC160.11557353366079169No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCAT160.11557353366079169No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTC160.11557353366079169No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAGTCGCGAGATGTCCCTCTT150.10835018780699221No Hit
GATCAATCATCACAGGGTTGGACGTAGAGGCCCTTGCGAGCAGGGTTGAC150.10835018780699221No Hit
GCATCAATCATCATGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG140.10112684195319273No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)