FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8491
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT379644.706159462960784No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT136116.028736309033093No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGCCATGTTGTAGAA4545.346837828288777No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG520.6124131433282298No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG400.4710870333294076No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGCTGTCCCTCTT360.4239783299964668No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT350.4122011541632316No Hit
GCATCAATCTTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT340.40042397832999643No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAACTCGCGAGATGTCCCTCTT290.3415380991638205No Hit
GCATCAATCATCCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT290.3415380991638205No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG280.3297609233305853No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTCCATGTTGTAGAACCA270.31798374749735014No Hit
GCATCAATCCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT240.28265221999764456No Hit
GCATCAATCATCACCGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT230.2708750441644094No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG230.2708750441644094No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG200.2355435166647038No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT200.2355435166647038No Hit
GCATCAATCATCACAGGGGTGGACTCGAGAAATCGCGAGATGTCCCTCTT180.2119891649982334No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCACTT180.2119891649982334No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTA180.2119891649982334No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGCCCCTCTT170.20021198916499822No Hit
GCATAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG170.20021198916499822No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCAT160.18843481333176304No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTGTT150.17665763749852784No Hit
GCCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT150.17665763749852784No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG150.17665763749852784No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCATCTT150.17665763749852784No Hit
ACGTTGCCTTTATCCTCCCACAGAGCCTTGGCATGCTCATAGAATTCGGG140.16488046166529266No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTC140.16488046166529266No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGGTGTCCCTCTT130.15310328583205746No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT130.15310328583205746No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG130.15310328583205746No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG130.15310328583205746No Hit
GCATCAATCATCACAGGGTTGGACTCGAGATATCGCGAGATGTCCCTCTT130.15310328583205746No Hit
GCATCAATCATCACAGGGTTGGGCTCGAGAAATCGCGAGATGTCCCTCTT130.15310328583205746No Hit
GCATCAATCATCATGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG120.14132610999882228No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.12954893416558708No Hit
GCATCAAACATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT110.12954893416558708No Hit
GCTCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.12954893416558708No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAGTCGCGAGATGTCCCTCTT110.12954893416558708No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCCCTT110.12954893416558708No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT110.12954893416558708No Hit
GCATCAATCATCACAGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG110.12954893416558708No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGAAGTCCCTCTT110.12954893416558708No Hit
GCATCAATCATCACAGGGTTGGACTCGGGAAATCGCGAGATGTCCCTCTT100.1177717583323519No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTACCTCTT100.1177717583323519No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG100.1177717583323519No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT100.1177717583323519No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGACGTCCCTCTT100.1177717583323519No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGCCATGTTGTAGAA100.1177717583323519No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT100.1177717583323519No Hit
GCATCAATCATCACAGGGTTGGACACGAGAAATCGCGAGATGTCCCTCTT100.1177717583323519No Hit
GCATCACTCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT100.1177717583323519No Hit
CATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG90.1059945824991167No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)