FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13310
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG1061979.78211870773855No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTCACAGAGACCAA4373.2832456799398946No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC1290.9691960931630353No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG790.5935386927122465No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG740.5559729526671675No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG570.42824943651389935No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG530.3981968444778362No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG500.3756574004507889No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTTCACAGAGACCAAGTC500.3756574004507889No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG480.3606311044327573No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG410.3080390683696469No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG410.3080390683696469No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG380.2854996243425995No Hit
AGATCATGTACGTCGGGGACATCCGCAGCGTCACACAGAAGCATATCCAG360.270473328324568No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG360.270473328324568No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG360.270473328324568No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACGCAGAAGCATATCCAG350.26296018031555224No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG330.24793388429752067No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG300.2253944402704733No Hit
AGATCATGTACGTCGGGGACGCCCGCAGCGTCACACAGAAGCATATCCAG300.2253944402704733No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG300.2253944402704733No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCGTATCCAG290.21788129226145755No Hit
AGATCAGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG290.21788129226145755No Hit
AGATCATGTACGTGGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG270.202854996243426No Hit
AGATCATGACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG260.19534184823441023No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACGGAAGCATATCCAG250.18782870022539444No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGTATATCCAG250.18782870022539444No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAGGCATATCCAG240.18031555221637866No Hit
AGTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG240.18031555221637866No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG220.1652892561983471No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATACCCAG220.1652892561983471No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAACATATCCAG210.15777610818933133No Hit
AGATCATGTCGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG210.15777610818933133No Hit
GATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG210.15777610818933133No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGGAGCATATCCAG200.15026296018031557No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCGG200.15026296018031557No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCATCACACAGAAGCATATCCAG170.1277235161532682No Hit
AATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG170.1277235161532682No Hit
AGATCATGTACGTCGGGGACGTCTGCAGCGTCACACAGAAGCATATCCAG160.12021036814425246No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATGTCCAG140.10518407212622088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)