FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5665
Total Bases820.5 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAA183932.46248896734333No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC98117.31685789938217No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG91216.098852603706973No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTC4948.720211827007944No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCTGCATAGACCAA1903.3539276257722856No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTCT560.9885260370697264No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCTGCATAGACCAAG350.617828773168579No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCCTGCATAGACCAAG300.529567519858782No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG290.5119152691968226No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCCTGCATAGACCAA200.353045013239188No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCTGCATAGACCAAG190.3353927625772286No Hit
CCATTAGTAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG190.3353927625772286No Hit
CCTTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG170.3000882612533098No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCCTGCATAGACCAAG170.3000882612533098No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG170.3000882612533098No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC160.2824360105913504No Hit
CCATTAGTGAGCTGGCCAACCCTGGATGATTGATGCCTGCATAGACCAAG150.264783759929391No Hit
CCATTAGTGAGCGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG130.2294792586054722No Hit
AGATCATGTACGTCGGGGATAAAGGCAACGTCTGCATAGACCAAGTCTCT130.2294792586054722No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCCTGCATAGACCAAG120.2118270079435128No Hit
CCATAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG120.2118270079435128No Hit
CCATTATGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG110.1941747572815534No Hit
CCATTAGTGAGCTGGCCAACCCTGTGAGATTGATGCCTGCATAGACCAAG110.1941747572815534No Hit
CCATTGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG110.1941747572815534No Hit
CCATTATGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG100.176522506619594No Hit
CCATTAGTGACTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG100.176522506619594No Hit
AGATCATGTACGTCGGGGAGGATAAAAGGCAACGTCTGCATAGACCAAGT100.176522506619594No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.1588702559576346No Hit
AGATCATGTACGTCGAGGATAAAGGCAACGTCTGCATAGACCAAGTCTCT90.1588702559576346No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGAGCCTGCATAGACCAAG90.1588702559576346No Hit
CCATTAGGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.1588702559576346No Hit
CCATTAGTGAGCTGGCCAACCCGTGATGATTGATGCCTGCATAGACCAAG90.1588702559576346No Hit
CCATTAGTGAGCTGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG90.1588702559576346No Hit
CCATTAGTGAGCTGGCCAACCTGTGATGATTGATGCCTGCATAGACCAAG90.1588702559576346No Hit
CCATTAGTGGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG90.1588702559576346No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCTGCATAGACCAA80.1412180052956752No Hit
CCATTAGTGAGCTGGCCACCCTGTGATGATTGATGCCTGCATAGACCAAG80.1412180052956752No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATCCTGCATAGACCAAG80.1412180052956752No Hit
CCATTAGTGAGCTGGCCAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.1235657546337158No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG70.1235657546337158No Hit
CCATTAGTGAGTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG70.1235657546337158No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATATTGATGCCTGCATAGACCAAG70.1235657546337158No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAAGTCTGCATAGACCAAGTCT70.1235657546337158No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACAAA70.1235657546337158No Hit
CCATTAGTGACTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.1235657546337158No Hit
CCATTAGTAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.1235657546337158No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTGCATAGACCAAG60.1059135039717564No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGTTGGCTATACCTCGAGAAATC60.1059135039717564No Hit
CATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCTGCATAGACCAAG60.1059135039717564No Hit
CCATTAGTGAGTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG60.1059135039717564No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG60.1059135039717564No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG60.1059135039717564No Hit
CCATTAGTGAGCTGGTCAACCCTGTGATGATTGATGCCTGCATAGACCAA60.1059135039717564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)