FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3843
Total Bases555 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT116130.210772833723652No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA116030.18475149622691No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA3228.378870673952642No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA721.873536299765808No Hit
ACGTTGCCTTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGG320.8326827998959146No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGAATCCTGTAGAACCAT270.702576112412178No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT230.5984907624251886No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC170.44236273744470467No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC160.4163413999479573No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC130.33827738745771535No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG120.312256049960968No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC110.28623471246422066No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGAATCCTGTAGAAC100.2602133749674733No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGAATCCTGTAGAA100.2602133749674733No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT90.234192037470726No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC90.234192037470726No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC90.234192037470726No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC90.234192037470726No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC90.234192037470726No Hit
ACGTTGCCTTTATCCCCGACGTACATGATCTGAATCCTGTAGAACCATGT80.20817069997397866No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG80.20817069997397866No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC80.20817069997397866No Hit
ACGTTGCCTTTATCCTCCCACAGAGCCTTGGCATGCTCATAGAATTCGGG70.18214936247723132No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGAATCCTGTAGAAC70.18214936247723132No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT70.18214936247723132No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.18214936247723132No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.18214936247723132No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.156128024980484No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.156128024980484No Hit
GCATCAATCATCACAGGGTTGGCCGCTCACTAATGGGAATCCTGTAGAAC60.156128024980484No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGAATCCTGTAGAAC60.156128024980484No Hit
ACGTTGCCTTTAACCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.156128024980484No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCACTCGATCTGGT60.156128024980484No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGAATCCTGTAGAAC60.156128024980484No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGAATCCTGTAGAAC50.13010668748373666No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT50.13010668748373666No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.13010668748373666No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGACCCATTCGATCTGGT50.13010668748373666No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGAATCCTGTAGAAC50.13010668748373666No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC50.13010668748373666No Hit
ACGTTGCCTTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACC50.13010668748373666No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT50.13010668748373666No Hit
GCATCAATCATCACAGGGTTGGCCAGTCACTAATGGGAATCCTGTAGAAC50.13010668748373666No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.13010668748373666No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.13010668748373666No Hit
ACATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA40.10408534998698933No Hit
GCATCAATCATCACGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.10408534998698933No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGTCTGAATCCTGTAGAACCAT40.10408534998698933No Hit
ACGTTGCCTTTATCCTCCCAGATCCTGGAGTGGGGCCCATTCGATCTGGT40.10408534998698933No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCTCATTCGATCTGGT40.10408534998698933No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGCCCATTCGATCTGGTG40.10408534998698933No Hit
CATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.10408534998698933No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCTTTCGATCTGGT40.10408534998698933No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCGATTCGATCTGGT40.10408534998698933No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT40.10408534998698933No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTAGATCTGGT40.10408534998698933No Hit
GCATCAATCACACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.10408534998698933No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.10408534998698933No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)