FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4125
Total Bases597.3 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAA128531.151515151515152No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC82119.903030303030302No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG78318.981818181818184No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTC2586.254545454545454No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCTGCATAGACCAA952.3030303030303028No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTCT310.7515151515151516No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG220.5333333333333333No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTGCATAGACCAAG180.4363636363636364No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC160.3878787878787879No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCCTGCATAGACCAAG160.3878787878787879No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCTGCATAGACCAAG150.36363636363636365No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATCCTGCATAGACCAAG140.3393939393939394No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCTGCATAGACCAA130.3151515151515152No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCCTGCATAGACCAAG130.3151515151515152No Hit
CCATTAGTGAGCTGGCCAACCCTGGATGATTGATGCCTGCATAGACCAAG110.26666666666666666No Hit
CCATTAGTGAGCTGGCCAACCCTGTGAGATTGATGCCTGCATAGACCAAG100.24242424242424243No Hit
AGATCATGTACGTCGGGGAGGATAAAAGGCAACGTCTGCATAGACCAAGT100.24242424242424243No Hit
CCTTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG90.2181818181818182No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG90.2181818181818182No Hit
CCATTAGTGAGTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG90.2181818181818182No Hit
CCATTGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG90.2181818181818182No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCTGCATAGACCAAG80.19393939393939394No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.19393939393939394No Hit
CCATTAGTAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG80.19393939393939394No Hit
CCATTATGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG80.19393939393939394No Hit
CCATTAGTGAGCTGGCCAACCCTGTGTGATTGATGCCTGCATAGACCAAG80.19393939393939394No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCCTGCATAGACCAAG70.1696969696969697No Hit
CCATTAGGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCA70.1696969696969697No Hit
CCATTAGTGAGCTGGCCAACCCGTGATGATTGATGCCTGCATAGACCAAG70.1696969696969697No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG60.14545454545454545No Hit
CCATTAGTGACTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG60.14545454545454545No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGAGCCTGCATAGACCAAG60.14545454545454545No Hit
CCATTGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCA60.14545454545454545No Hit
AGATCATGTACGTCGGGGATAAAGGCAACGTCTGCATAGACCAAGTCTCT60.14545454545454545No Hit
CCATTAGTGGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG60.14545454545454545No Hit
CCTTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCA50.12121212121212122No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCCTGCATAGACCAA50.12121212121212122No Hit
AGATCATGTACGTCGAGGATAAAGGCAACGTCTGCATAGACCAAGTCTCT50.12121212121212122No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATATTGATGCCTGCATAGACCAAG50.12121212121212122No Hit
CCATTAGTGAGCTGGCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCA50.12121212121212122No Hit
CCATTAGGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG50.12121212121212122No Hit
CCATTAGTGAGCTGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG50.12121212121212122No Hit
CCATTAGTGAGCTGGCCAACCTGTGATGATTGATGCCTGCATAGACCAAG50.12121212121212122No Hit
CCATAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG50.12121212121212122No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.12121212121212122No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)