FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438
Total Bases353.4 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAA108244.38063986874488No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG34614.191960623461854No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTC27911.44380639868745No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCTGCATAGACCAA833.4044298605414274No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC833.4044298605414274No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG271.1074651353568499No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTCT251.0254306808859721No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCTGCATAGACCAAG150.6152584085315832No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTGCATAGACCAAG130.5332239540607054No Hit
CCATTAGTGAGCTGGCCAACCCTGTGAGATTGATGCCTGCATAGACCAAG130.5332239540607054No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCCTGCATAGACCAAG110.4511894995898278No Hit
CCATAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG110.4511894995898278No Hit
CCATTAGTGAGCTGGCCAACCCTGGATGATTGATGCCTGCATAGACCAAG100.41017227235438886No Hit
AGATCATGTACGTCGGAGGATAAAGGCAACGTCTGCATAGACCAAGTCTC90.3691550451189499No Hit
CCATTAGTAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG80.3281378178835111No Hit
CCATTATGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG80.3281378178835111No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCCTGCATAGACCAA80.3281378178835111No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATCCTGCATAGACCAAG80.3281378178835111No Hit
CCTTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG70.2871205906480722No Hit
CCATTGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG70.2871205906480722No Hit
AGATCATGTACGTCGGGGAGGATAAAAGGCAACGTCTGCATAGACCAAGT70.2871205906480722No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCTGCATAGACCAA60.2461033634126333No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCCTGCATAGACCAAG60.2461033634126333No Hit
CCATTAGTGAGCTGGCCAACCTGTGATGATTGATGCCTGCATAGACCAAG60.2461033634126333No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCCTGCATAGACCAAG60.2461033634126333No Hit
CCATTAGTGAGCTGGCCACCCTGTGATGATTGATGCCTGCATAGACCAAG60.2461033634126333No Hit
CCATTAGTGAGTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG60.2461033634126333No Hit
CCATTAGTGGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG60.2461033634126333No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.20508613617719443No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATATTGATGCCTGCATAGACCAAG50.20508613617719443No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.20508613617719443No Hit
CCATTAGTGAGCGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG50.20508613617719443No Hit
AGATCATGTACGTCGGGGATAAAGGCAACGTCTGCATAGACCAAGTCTCT50.20508613617719443No Hit
CCATTAGTGAGCTGTCCAACCCTGTGATGATTGATGCCTGCATAGACCAA50.20508613617719443No Hit
AGATCATGTACGTCGAGGATAAAGGCAACGTCTGCATAGACCAAGTCTCT40.16406890894175555No Hit
CCATTAGGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG40.16406890894175555No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGAGCCTGCATAGACCAAG40.16406890894175555No Hit
AGATCATGTACTCGGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTCT30.12305168170631665No Hit
CCATTAGTGACTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG30.12305168170631665No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCTGCATAGACCAAG30.12305168170631665No Hit
CCATTAGTGAGCTGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG30.12305168170631665No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG30.12305168170631665No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG30.12305168170631665No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTAATGCCTGCATAGACCAA30.12305168170631665No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGTGCCTGCATAGACCAAG30.12305168170631665No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCACGTCTGCATAGACCAAGTCT30.12305168170631665No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)