FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3499
Total Bases505.4 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC60

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA120734.49557016290369No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT65518.71963418119463No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA3048.688196627607887No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT2126.058873963989711No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA912.6007430694484137No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGAATCCTGTAGAACCAT300.8573878250928837No Hit
ACGTTGCCTTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGG210.6001714775650185No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC190.5430122892254929No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC170.48585310088596745No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC140.40011431837667905No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT130.3715347242069163No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC110.3143755358673907No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC110.3143755358673907No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGAATCCTGTAGAAC100.2857959416976279No Hit
ACGTTGCCTTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACC100.2857959416976279No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.2857959416976279No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC100.2857959416976279No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGAATCCTGTAGAAC90.2572163475278651No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGAATCCTGTAGAAC80.22863675335810232No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC80.22863675335810232No Hit
CATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC80.22863675335810232No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGAATCCTGTAGAAC70.20005715918833952No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT70.20005715918833952No Hit
ACGTTGCCTTTATCCTCCGACGTACATGATCTGAATCCTGTAGAACCATG70.20005715918833952No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.17147756501857675No Hit
GCATCAATCATCACGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.17147756501857675No Hit
ACGTTGCCTTTATCCCCCCGACGTACATGATCTGAATCCTGTAGAACCAT60.17147756501857675No Hit
ACGTTGCCTTTATCCCCGACGTACATGATCTGAATCCTGTAGAACCATGT60.17147756501857675No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGAATCCTGTAGAAC60.17147756501857675No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.14289797084881395No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGAATCCTGTAGAAC50.14289797084881395No Hit
GCATCAATCATCACAGGGTTGGCCAACTCACTAATGGGAATCCTGTAGAA50.14289797084881395No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.14289797084881395No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAC50.14289797084881395No Hit
GCACAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.14289797084881395No Hit
GCATCAATCATCACAGGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGA50.14289797084881395No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.14289797084881395No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCCTAATGGGAATCCTGTAGAAC50.14289797084881395No Hit
AGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCAT40.11431837667905116No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.11431837667905116No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGAATCCTGTAGAAC40.11431837667905116No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.11431837667905116No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.11431837667905116No Hit
GCATCAATCATCACCGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA40.11431837667905116No Hit
GCATCAATCATCACAGGGTTGACCAGCTCACTAATGGGAATCCTGTAGAA40.11431837667905116No Hit
GCATCAATCATCACAGGGTTGGCCAGCTAACTAATGGGAATCCTGTAGAA40.11431837667905116No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGACCCATTCGATCTGGT40.11431837667905116No Hit
ACGTTGCCTTTATCCTCCCTGATCCAGGAGTGGGGCCCATTCGATCTGGT40.11431837667905116No Hit
GCATCAATCATCACAGGGTTGGTCAGCTCACTAATGGGAATCCTGTAGAA40.11431837667905116No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGAATCCTGTAGAA40.11431837667905116No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT40.11431837667905116No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.11431837667905116No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)