FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542
Total Bases368 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC60

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA102740.40125885129819No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT54421.400472069236823No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA1515.940204563335956No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA652.5570416994492526No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT391.5342250196695515No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC180.7081038552321007No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGAATCCTGTAGAACCAT170.6687647521636507No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC160.6294256490952006No Hit
ACGTTGCCTTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGG150.5900865460267506No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGAATCCTGTAGAAC120.47206923682140045No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGAATCCTGTAGAAC100.3933910306845004No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC90.35405192761605037No Hit
GCATCAATCATCACAGGGTTGGCCAGTCACTAATGGGAATCCTGTAGAAC90.35405192761605037No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC80.3147128245476003No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGAATCCTGTAGAAC80.3147128245476003No Hit
GCATCAATCATCACGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.2753737214791503No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGAATCCTGTAGAA70.2753737214791503No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.2753737214791503No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.2753737214791503No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA60.23603461841070023Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.23603461841070023No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC60.23603461841070023No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.23603461841070023No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCCTAATGGGAATCCTGTAGAAC60.23603461841070023No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGAATCCTGTAGAAC60.23603461841070023No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.1966955153422502No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.1966955153422502No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGAATCCTGTAGAAC50.1966955153422502No Hit
GCATCAATCATCACCGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA50.1966955153422502No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.1966955153422502No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGAATCCTGTAGAAC50.1966955153422502No Hit
ACGTTGCCTTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACC50.1966955153422502No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.1966955153422502No Hit
GCATCAATCATCACAGGGTTGGCCGCTCACTAATGGGAATCCTGTAGAAC40.15735641227380015No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCAGTAGAA40.15735641227380015No Hit
GCATCAATCACACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.15735641227380015No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.11801730920535011No Hit
ACATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA30.11801730920535011No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGG30.11801730920535011No Hit
GCATCAATCATCACAGGGTTGGCCAGCACACTAATGGGAATCCTGTAGAA30.11801730920535011No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG30.11801730920535011No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCGTTCGATCTGGT30.11801730920535011No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGAATCCTGTAGAAC30.11801730920535011No Hit
GCATCATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.11801730920535011No Hit
ACGTTGCCTTTATCCTCCCACAGAGCCTTGGCATGCTCATAGAATTCGGG30.11801730920535011No Hit
ACGTTGCCTTTAACCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT30.11801730920535011No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGAAGAA30.11801730920535011No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCGATTCGATCTGGT30.11801730920535011No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGCCGCCTGTAGAA30.11801730920535011No Hit
GCATCAATCATCACAGGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGA30.11801730920535011No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG30.11801730920535011No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.11801730920535011No Hit
GCATCAATCATCACAGGGTTGGCCAGCTTACTAATGGGAATCCTGTAGAA30.11801730920535011No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.11801730920535011No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)