FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008130997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008130997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5089
Total Bases737 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA168633.130280998231484No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT155230.497150717233247No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA3186.2487718608764No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA931.8274710159166832No Hit
ACGTTGCCTTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGG350.6877579092159559No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGAATCCTGTAGAACCAT310.609157005305561No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC280.5502063273727648No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT240.4716054234623698No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC210.41265474552957354No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG170.3340538416191786No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC160.31440361564157987No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG160.31440361564157987No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG150.2947533896639811No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC150.2947533896639811No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC130.25545293770878363No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGAATCCTGTAGAAC120.2358027117311849No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC120.2358027117311849No Hit
ACGTTGCCTTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACC120.2358027117311849No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGAATCCTGTAGAA110.21615248575358614No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC110.21615248575358614No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC110.21615248575358614No Hit
GCATCAATCATCACAGGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGA110.21615248575358614No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGAATCCTGTAGAAC100.19650225977598745No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC100.19650225977598745No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC90.17685203379838868No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGAATCCTGTAGAAC90.17685203379838868No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGAATCCTGTAGAAC90.17685203379838868No Hit
CATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC90.17685203379838868No Hit
ACGTTGCCTTTATCCCCGACGTACATGATCTGAATCCTGTAGAACCATGT90.17685203379838868No Hit
GCATCAATCATCACGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC80.15720180782078993No Hit
GCATCAATCATCACAGGGTTGACCAGCTCACTAATGGGAATCCTGTAGAA80.15720180782078993No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGAATCCTGTAGAAC80.15720180782078993No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC80.15720180782078993No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT70.1375515818431912No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.1375515818431912No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT70.1375515818431912No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.11790135586559244No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGAATCCTGTAGAAC60.11790135586559244No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.11790135586559244No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGGGTGGGGCCCATTCGATCTGGT60.11790135586559244No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.11790135586559244No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT60.11790135586559244No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT60.11790135586559244No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGAATCCTGTAGAAC60.11790135586559244No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT60.11790135586559244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)