FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2053
Total Bases297.6 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAA86241.98733560642962No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG55126.838772528007794No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTC1467.111544081831466No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCTGCATAGACCAA592.8738431563565516No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCTGCATAGACCAAG150.7306380905991232No Hit
CCATTAGTAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG130.6332196785192401No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG120.5845104724792985No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTCT120.5845104724792985No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCTGCATAGACCAAG110.535801266439357No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCCTGCATAGACCAAG110.535801266439357No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCTGCATAGACCAA80.3896736483195324No Hit
CCATAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG80.3896736483195324No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCCTGCATAGACCAA70.34096444227959083No Hit
CCATTAGTGAGCTGGCCAACCTGTGATGATTGATGCCTGCATAGACCAAG70.34096444227959083No Hit
CCATTAGTGAGCTGGCCAACCCTGGATGATTGATGCCTGCATAGACCAAG70.34096444227959083No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATATTGATGCCTGCATAGACCAAG60.2922552362396493No Hit
CCTTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG60.2922552362396493No Hit
CCATTAGTGAGCTGGCCAACCCTGTGTGATTGATGCCTGCATAGACCAAG60.2922552362396493No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.24354603019970775No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTGCATAGACCAAG50.24354603019970775No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.24354603019970775No Hit
CCATTGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG50.24354603019970775No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.24354603019970775No Hit
CCATTAGTGAGTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG50.24354603019970775No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG40.1948368241597662No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACGGAAGCATATCCAG40.1948368241597662No Hit
AGATCATGTACGTCGGGGAGGATAAAAGGCAACGTCTGCATAGACCAAGT40.1948368241597662No Hit
AGATCATGTACGTCGGGGATAAAGGCAACGTCTGCATAGACCAAGTCTCT40.1948368241597662No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG40.1948368241597662No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATACCCAG40.1948368241597662No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATCCTGCATAGACCAAG40.1948368241597662No Hit
AGATCATATACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG30.14612761811982464No Hit
AGATCATGTCGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG30.14612761811982464No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCCTGCATAGACCAAG30.14612761811982464No Hit
CCATTATGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG30.14612761811982464No Hit
CCATTAGTGAGCTGGCCAACCCTGTGAGATTGATGCCTGCATAGACCAAG30.14612761811982464No Hit
AGATCATGTACGTGGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG30.14612761811982464No Hit
AGTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG30.14612761811982464No Hit
CCATTAGGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG30.14612761811982464No Hit
AGATCATGTACGTCGGGGGGATAAAGGCAACGTCTGCATAGACCAAGTCT30.14612761811982464No Hit
CCATTAGTGAGCTGGCCAACCCGTGATGATTGATGCCTGCATAGACCAAG30.14612761811982464No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG30.14612761811982464No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCAAAGACCAA30.14612761811982464No Hit
AGATCATGACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG30.14612761811982464No Hit
AGATCATGTACGTCGGAGGATAAAGGCAACGTCTGCATAGACCAAGTCTC30.14612761811982464No Hit
AGATCATGTACGTCGGGGAGGATAAAGCAACGTCTGCATAGACCAAGTCT30.14612761811982464No Hit
CCATTAGTGGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG30.14612761811982464No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG30.14612761811982464No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)