FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430
Total Bases207.3 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA81957.27272727272727No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA15010.48951048951049No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA584.055944055944056No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC221.5384615384615385No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGAATCCTGTAGAACCAT120.8391608391608392No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC110.7692307692307693No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC90.6293706293706294No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGAATCCTGTAGAAC80.5594405594405595No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGAATCCTGTAGAAC70.48951048951048953No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.48951048951048953No Hit
ACGTTGCCTTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACC60.4195804195804196No Hit
GCATCAATCATCACGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.4195804195804196No Hit
GCATCAATCATCACAGGGTTGGCCAGTCACTAATGGGAATCCTGTAGAAC60.4195804195804196No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.34965034965034963No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGAATCCTGTAGAAC50.34965034965034963No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC50.34965034965034963No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.34965034965034963No Hit
GCATCAATCATCACAGGGTTGGCCGCTCACTAATGGGAATCCTGTAGAAC50.34965034965034963No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCCTAATGGGAATCCTGTAGAAC50.34965034965034963No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGAATCCTGTAGAAC50.34965034965034963No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.34965034965034963No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.27972027972027974No Hit
ACGTTGCCTTTATCCCCCCGACGTACATGATCTGAATCCTGTAGAACCAT40.27972027972027974No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.27972027972027974No Hit
ACGTTGCCTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCAT40.27972027972027974No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.27972027972027974No Hit
ACGTTGCCTTTATCCCCCGACGTACATGATCTGAATCCTGTAGAACCATG30.2097902097902098No Hit
GCATCAACATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.2097902097902098No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.2097902097902098No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGAATCCTGTAGAA30.2097902097902098No Hit
GCATCAATAATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA30.2097902097902098No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.2097902097902098No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAC30.2097902097902098No Hit
GCACAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.2097902097902098No Hit
GCATCAATCATCACAGGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGA30.2097902097902098No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGAATCCTGTAGAAC30.2097902097902098No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGAATCCTGTAGAAC30.2097902097902098No Hit
GCATCAATCATCACAGGGCTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.13986013986013987No Hit
ACATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.13986013986013987No Hit
ACGTTGCCTTTATCTCCCCGACGTACATGATCTGAATCCTGTAGAACCAT20.13986013986013987No Hit
CCGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA20.13986013986013987No Hit
GCATTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGA20.13986013986013987No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTATAA20.13986013986013987No Hit
CATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.13986013986013987No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGAATCCTGTAGAAC20.13986013986013987No Hit
ACGTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCAT20.13986013986013987No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGTT20.13986013986013987No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGCA20.13986013986013987No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTTGGGAATCCTGTAGAACC20.13986013986013987No Hit
GCAGCAATCATCACTGGGTTGGGGTGGTGACTAATGGGTATCCTGTAGAA20.13986013986013987No Hit
ACGTTGCCTTTATCGTCCCCGACGTACATGATCTGAATCCTGTAGAACCA20.13986013986013987No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCCTAATGGGAATCCTGTAGAAC20.13986013986013987No Hit
GCATCAATCACACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.13986013986013987No Hit
ACGTTGCCTTTATCCTCCGACGTACATGATCTGAATCCTGTAGAACCATG20.13986013986013987No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAAACCTGTAGAA20.13986013986013987No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.13986013986013987No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)