FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6573
Total Bases952.5 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG276142.00517267609919No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAA162124.66149399056747No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC5868.91525939449262No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTC2744.168568385820782No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCTGCATAGACCAA1352.0538566864445458No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG250.38034383082306406No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTCT230.3499163243572189No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCTGCATAGACCAAG200.3042750646584512No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG190.2890613114255287No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG170.2586338049596835No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG170.2586338049596835No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCTGCATAGACCAAG160.24342005172676098No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCCTGCATAGACCAAG150.22820629849383844No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCTGCATAGACCAA140.21299254526091588No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC140.21299254526091588No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCCTGCATAGACCAAG140.21299254526091588No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG120.18256503879507074No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG120.18256503879507074No Hit
AGATCATGACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG120.18256503879507074No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATCCTGCATAGACCAAG120.18256503879507074No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCCTGCATAGACCAAG110.16735128556214818No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG110.16735128556214818No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG110.16735128556214818No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG110.16735128556214818No Hit
CCATTAGTAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG110.16735128556214818No Hit
CCATTAGTGAGCTGGCCAACCCTGGATGATTGATGCCTGCATAGACCAAG110.16735128556214818No Hit
CCATTAGTGAGCTGGCCAACCTGTGATGATTGATGCCTGCATAGACCAAG100.1521375323292256No Hit
CCATAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG100.1521375323292256No Hit
CCTTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG90.13692377909630307No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCCTGCATAGACCAA90.13692377909630307No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATATTGATGCCTGCATAGACCAAG90.13692377909630307No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCGTATCCAG90.13692377909630307No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG90.13692377909630307No Hit
AATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.13692377909630307No Hit
AGATCATGTCGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.12171002586338049No Hit
AGATCATGTACGTGGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG80.12171002586338049No Hit
AGTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.12171002586338049No Hit
GATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.12171002586338049No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCACATCCAG80.12171002586338049No Hit
CCATTAGTGACTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG80.12171002586338049No Hit
CCATTGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG80.12171002586338049No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.12171002586338049No Hit
CCATTAGTGGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG80.12171002586338049No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG70.10649627263045794No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCCCACAGAAGCATATCCAG70.10649627263045794No Hit
CCATTAGTGAGTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG70.10649627263045794No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG70.10649627263045794No Hit
CCATTATGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG70.10649627263045794No Hit
AGATCATGTACGTCGGGGACGTCCACAGCGTCACACAGAAGCATATCCAG70.10649627263045794No Hit
CCATTAGTGAGCTGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG70.10649627263045794No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG70.10649627263045794No Hit
AGATCATGTACGTCGGGGACGTCTGCAGCGTCACACAGAAGCATATCCAG70.10649627263045794No Hit
AGATCAGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG70.10649627263045794No Hit
AGATCATGTACGTCGGGGACGTCCGCAGTGTCACACAGAAGCATATCCAG70.10649627263045794No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)