FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1654
Total Bases239.8 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA97058.64570737605804No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA1408.464328899637243No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA603.6275695284159615No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC221.3301088270858523No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC211.2696493349455864No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGAATCCTGTAGAACCAT181.0882708585247884No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGAATCCTGTAGAAC120.7255139056831923No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC100.6045949214026602No Hit
GCATCAATCATCACAGGGTTGGCCAGTCACTAATGGGAATCCTGTAGAAC90.5441354292623942No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGAATCCTGTAGAAC90.5441354292623942No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.42321644498186217No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGAATCCTGTAGAAC70.42321644498186217No Hit
GCATCAATAATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA70.42321644498186217No Hit
GCATCAATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTA60.36275695284159615Clontech SMARTer II A Oligonucleotide (100% over 24bp)
ACGTTGCCTTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACC60.36275695284159615No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGAATCCTGTAGAAC60.36275695284159615No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGAATCCTGTAGAAC60.36275695284159615No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.3022974607013301No Hit
GCATCAATCATCACGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.3022974607013301No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.3022974607013301No Hit
GCATCAATCATCACAGGGTACATGGAAGCAGTGGTATCAACGCAGAGTAC50.3022974607013301Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.3022974607013301No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.3022974607013301No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC40.24183796856106407No Hit
CATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.24183796856106407No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGAATCCTGTAGAAC40.24183796856106407No Hit
ACGTTGCCTTTATCCCCGACGTACATGATCTGAATCCTGTAGAACCATGT40.24183796856106407No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGAATCCTGTAGAA40.24183796856106407No Hit
GCATCAATCATCACAGGGTTGGCCGCTCACTAATGGGAATCCTGTAGAAC40.24183796856106407No Hit
GCATCAATCATCACAGGGTTGGCCAGCTAACTAATGGGAATCCTGTAGAA40.24183796856106407No Hit
GCATCAATCACACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.24183796856106407No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.24183796856106407No Hit
GCATCAATCATCACAGGGTTGGCCAGCCCACTAATGGGAATCCTGTAGAA40.24183796856106407No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.18137847642079807No Hit
GCATCAATCATCACAGGGTTGGCCAGCACACTAATGGGAATCCTGTAGAA30.18137847642079807No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.18137847642079807No Hit
GCATCAATCATCACCGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA30.18137847642079807No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.18137847642079807No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCCTAATGGGAATCCTGTAGAAC30.18137847642079807No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGAATCCTGTAGAAC30.18137847642079807No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.18137847642079807No Hit
GCATCAATTATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.12091898428053204No Hit
GCATCAATCATCACAGGCTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.12091898428053204No Hit
ACATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.12091898428053204No Hit
GCATCAATCATCACAGGGTTGGTCAGCTCACTAATGGGAATCCTGTAGAA20.12091898428053204No Hit
GCCGCCCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.12091898428053204No Hit
ACGTTGCCTTTATCCCCCGACGTACATGATCTGAATCCTGTAGAACCATG20.12091898428053204No Hit
GCATCAAACATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.12091898428053204No Hit
GCATAAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.12091898428053204No Hit
GCATCAACATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.12091898428053204No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGAATCCTGTAGAACC20.12091898428053204No Hit
GCATCAATCATCACAGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.12091898428053204No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT20.12091898428053204No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATCGGAATCCTGTAGAA20.12091898428053204No Hit
GCATCAATCATCACAGGGTTGGGCAGCTCACTAATGGGAATCCTGTAGAA20.12091898428053204No Hit
GCATCATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAACC20.12091898428053204No Hit
GCATCATCATCACAGGGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC20.12091898428053204Clontech Universal Primer Mix Long (96% over 26bp)
ACGTTGCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCAT20.12091898428053204No Hit
GCATCAATCATCACAGGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGA20.12091898428053204No Hit
ACGTTGCCTTTATCCTCCCCGAGTACATGATCTGAATCCTGTAGAACCAT20.12091898428053204No Hit
GCATCAATCATCACAGGGTACATAGGAAGCAGTGGTATCAACGCAGAGTA20.12091898428053204Clontech Universal Primer Mix Long (96% over 25bp)
GCATCAATCATCACAGGGGTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.12091898428053204No Hit
ACGTTGCCTTTATCCTCCGACGTACATGATCTGAATCCTGTAGAACCATG20.12091898428053204No Hit
GCATCTATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.12091898428053204No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)