FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3735
Total Bases541.1 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAA135636.30522088353413No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG69918.714859437751006No Hit
CCATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATC50413.493975903614459No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTC2466.586345381526105No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCTGCATAGACCAA1233.2931726907630523No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG270.7228915662650602No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTCT240.642570281124498No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCCTGCATAGACCAAG220.5890227576974565No Hit
CCATTAGTAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG170.45515394912985274No Hit
CCATTAGTGGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG170.45515394912985274No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCTGCATAGACCAAG160.428380187416332No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCTGCATAGACCAAG130.34805890227576974No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTGCATAGACCAAG110.29451137884872824No Hit
CCATTGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG110.29451137884872824No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATATTGATGCCTGCATAGACCAAG100.2677376171352075No Hit
CCATTAGTGAGCTGGCCAACCCAAGGTTGCTGGCTATACCTCGAGAAATC100.2677376171352075No Hit
CCATTAGTGAGCGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG100.2677376171352075No Hit
CCATTAGTGAGTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG100.2677376171352075No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCCTGCATAGACCAA90.24096385542168677No Hit
CCATTAGTGAGCTGGCCAACCCTGTGAGATTGATGCCTGCATAGACCAAG90.24096385542168677No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCTGCATAGACCAA80.214190093708166No Hit
CCTTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG80.214190093708166No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.214190093708166No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG80.214190093708166No Hit
CCATTAGTGAGTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG80.214190093708166No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATCCTGCATAGACCAAG80.214190093708166No Hit
CCATTAGTGAGCTGGCCAACCCGTGATGATTGATGCCTGCATAGACCAAG70.18741633199464525No Hit
CCATTAGTGAGCTGGCCAACCTGTGATGATTGATGCCTGCATAGACCAAG70.18741633199464525No Hit
CCATTAGTGAGCTGGCCAACCCTGGATGATTGATGCCTGCATAGACCAAG70.18741633199464525No Hit
CATTAGTGAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG70.18741633199464525No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCCTGCATAGACCAAG70.18741633199464525No Hit
CCATTAGTGAGCTGGCCACCCTGTGATGATTGATGCCTGCATAGACCAAG70.18741633199464525No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG70.18741633199464525No Hit
CCATTAGTGAGCTGGCCAAACCCTGTGATGATTGATGCCTGCATAGACCA60.1606425702811245No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGAGCCTGCATAGACCAAG60.1606425702811245No Hit
AGATCATGTACGTCGGGGAGGATAAAAGGCAACGTCTGCATAGACCAAGT60.1606425702811245No Hit
CCATTAGTGAGCTGGCCAACCCTGTGTGATTGATGCCTGCATAGACCAAG60.1606425702811245No Hit
CCATTAGTGAGCTGGCCAACCCCGAGAACCAGTTCAGAGTGGACTACATC50.13386880856760375No Hit
CCATTAGTGACTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG50.13386880856760375No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGCCACACAGAAGCATATCCAG50.13386880856760375No Hit
AGATCATGTACGTCGGGGATAAAGGCAACGTCTGCATAGACCAAGTCTCT50.13386880856760375No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACAAA40.107095046854083No Hit
AGTCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG40.107095046854083No Hit
AGATCATGTACGTCGGGGGGATAAAGGCAACGTCTGCATAGACCAAGTCT40.107095046854083No Hit
GATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG40.107095046854083No Hit
CCATTAGTAGCTGGCCAAACCAAGGTTGCTGGCTATACCTCGAGAAATCG40.107095046854083No Hit
CATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCTGCATAGACCAAG40.107095046854083No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG40.107095046854083No Hit
CCATAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG40.107095046854083No Hit
AGATCATGTACGTCGGAGGATAAAGGCAACGTCTGCATAGACCAAGTCTC40.107095046854083No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG40.107095046854083No Hit
CCATTAGTGAGTGGCCAAACCTGTGATGATTGATGCCTGCATAGACCAAG40.107095046854083No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)