FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2699
Total Bases391.3 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAA97736.19859207113746No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG86331.974805483512412No Hit
AGATCATGTACGTCGGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTC1405.187106335679881No Hit
CCATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCTGCATAGACCAA1344.964801778436458No Hit
CCATTAGTGAGCTGGCAACCCTGTGATGATTGATGCCTGCATAGACCAAG160.5928121526491293No Hit
AGATCATGTACGTCGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTCT160.5928121526491293No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATAGATGCCTGCATAGACCAA130.48165987402741756No Hit
CATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG130.48165987402741756No Hit
CCATTAGTGAGCTGGCCAACCCTGTATGATTGATGCCTGCATAGACCAAG110.4075583549462764No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATGATGCCTGCATAGACCAAG100.3705075954057058No Hit
CCATTAGTGGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG100.3705075954057058No Hit
CCATTAGTGAGCTGGCCAACCCTTGATGATTGATGCCTGCATAGACCAAG90.33345683586513525No Hit
AGATCATGTACGTGGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAG90.33345683586513525No Hit
AGATCATGTACGTGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.33345683586513525No Hit
CCATAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG90.33345683586513525No Hit
AGATCATTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG90.33345683586513525No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATATTGATGCCTGCATAGACCAAG80.29640607632456467No Hit
CCATTAGTGAGCTGGCCAAACCCTGTGATGATTGATGCCTGCATAGACCA80.29640607632456467No Hit
CCATTAGTGAGTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG80.29640607632456467No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCGCACAGAAGCATATCCAG70.2593553167839941No Hit
CCATTAGTGAGCTGGCCAACCCTGGATGATTGATGCCTGCATAGACCAAG70.2593553167839941No Hit
CCTTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG60.2223045572434235No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCTGCATAGACCAAG60.2223045572434235No Hit
CCATTATGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG60.2223045572434235No Hit
AGATCATGTACGTCGGGGGGATAAAGGCAACGTCTGCATAGACCAAGTCT60.2223045572434235No Hit
AGATCATGTACGTCGGGGAGGATAAAAGGCAACGTCTGCATAGACCAAGT60.2223045572434235No Hit
CCATTAGTAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG50.1852537977028529No Hit
AGATCATGTACTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.1852537977028529No Hit
AGATCATGTACGTCGGGGCCGTCCGCAGCGTCACACAGAAGCATATCCAG50.1852537977028529No Hit
CCATTAGTGAGCTGGCAAACCCTGTGATGATTGATGCCTGCATAGACCAA50.1852537977028529No Hit
AGATCATGTACGTCGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.1852537977028529No Hit
CCATTAGTGAGCTGGCCAACCCGTGATGATTGATGCCTGCATAGACCAAG50.1852537977028529No Hit
CCATTAGTGAGCTGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG50.1852537977028529No Hit
AGATCATGTACGCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.1852537977028529No Hit
AGATCATGTACGTCGGGGACGCCCGCAGCGTCACACAGAAGCATATCCAG50.1852537977028529No Hit
AGATCATGTACGTCGGGGCGTCCGCAGCGTCACACAGAAGCATATCCAGG50.1852537977028529No Hit
AGATCATGTAGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG50.1852537977028529No Hit
AGATCATGTACGTCGGGGACGTCCGCGGCGTCACACAGAAGCATATCCAG40.14820303816228234No Hit
CCATTAGTGAGCTGGCCAACCCTGTGAGATTGATGCCTGCATAGACCAAG40.14820303816228234No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACAAA40.14820303816228234No Hit
CCATTAGTGAGCTGGCCAAACTGTGATGATTGATGCCTGCATAGACCAAG40.14820303816228234No Hit
CCATTGTGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG40.14820303816228234No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATGACTGCATAGACCAA40.14820303816228234No Hit
CCATTAGTGAGCGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG40.14820303816228234No Hit
CCATTAGTGAGCTGGCCAACCTGTGATGATTGATGCCTGCATAGACCAAG40.14820303816228234No Hit
CCATTAGAGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAA40.14820303816228234No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTATGCCTGCATAGACCAAG40.14820303816228234No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATACCCAG40.14820303816228234No Hit
AGATCATGTACGTCGAGGATAAAGGCAACGTCTGCATAGACCAAGTCTCT30.11115227862171174No Hit
AGATATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG30.11115227862171174No Hit
CCATTAGTGAGTTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAA30.11115227862171174No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAGGCATATCCAG30.11115227862171174No Hit
AGATCTGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGG30.11115227862171174No Hit
CCATTAGGAGCTGGCCAACCCTGTGATGATTGATGCCTGCATAGACCAAG30.11115227862171174No Hit
AGATCATGTACGTGGGGGAGGATAAAGGCAACGTCTGCATAGACCAAGTC30.11115227862171174No Hit
AGATCATGTACGTCGGGGACGTCCGCAGCGTCACACTGAAGCATATCCAG30.11115227862171174No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGAGCCTGCATAGACCAAG30.11115227862171174No Hit
CATTAGTGAGCTGGCCAAACCTGTGATGATTGATGCCTGCATAGACCAAG30.11115227862171174No Hit
AGATCATGTACGTCGGGGACGTCTGCAGCGTCACACAGAAGCATATCCAG30.11115227862171174No Hit
CCATTAGTGAGCTGGCCACCCTGTGATGATTGATGCCTGCATAGACCAAG30.11115227862171174No Hit
CCATTAGTGAGTGGCCAAACCTGTGATGATTGATGCCTGCATAGACCAAG30.11115227862171174No Hit
CCATTAGTGAGCTGGCCAACCCTGTGATGATTGATCCTGCATAGACCAAG30.11115227862171174No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)