FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4318
Total Bases623.1 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC60

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA131430.430754979157015No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT92721.4682723483094No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT4229.773043075497915No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA3016.970819823992589No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA912.1074571560907827No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGAATCCTGTAGAACCAT320.7410838351088467No Hit
ACGTTGCCTTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGG280.6484483557202408No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC210.4863362667901806No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC180.41685965724872626No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC170.39370078740157477No Hit
ACGTTGCCTTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACC160.37054191755442334No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT130.30106530801296894No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC120.2779064381658175No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGAATCCTGTAGAAC110.2547475683186661No Hit
CATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC100.2315886984715146No Hit
GCATCAATCATCACAGGGTTGGCCAGTCACTAATGGGAATCCTGTAGAAC100.2315886984715146No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCCTAATGGGAATCCTGTAGAAC100.2315886984715146No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGAATCCTGTAGAAC90.20842982862436313No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC90.20842982862436313No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT80.18527095877721167No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGGAATCCTGTAGAAC80.18527095877721167No Hit
GCATCAATCATCACGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC80.18527095877721167No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGAATCCTGTAGAAC80.18527095877721167No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC80.18527095877721167No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG80.18527095877721167No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC80.18527095877721167No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG80.18527095877721167No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.1621120889300602No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.1621120889300602No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.1621120889300602No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGAATCCTGTAGAAC70.1621120889300602No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.1621120889300602No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGAATCCTGTAGAAC70.1621120889300602No Hit
GCATTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGA60.13895321908290875No Hit
GCATCAATCATCACAGGGTTGGCCGCTCACTAATGGGAATCCTGTAGAAC60.13895321908290875No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGAATCCTGTAGAAC60.13895321908290875No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.13895321908290875No Hit
ACGTTGCCTTTATCCCCGACGTACATGATCTGAATCCTGTAGAACCATGT60.13895321908290875No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGAATCCTGTAGAA60.13895321908290875No Hit
GCATCAATCATCACAGGTTTGGCCACTCACTAATGGGAATCCTGTAGAAC50.1157943492357573No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG50.1157943492357573No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGAATCCTGTAGAAC50.1157943492357573No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGGTGTCCCTCTT50.1157943492357573No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.1157943492357573No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT50.1157943492357573No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.1157943492357573No Hit
GCATCAACATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.1157943492357573No Hit
ACGTTGCCTTTATCCTCGACGTACATGATCTGAATCCTGTAGAACCATGT50.1157943492357573No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.1157943492357573No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)