FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4971
Total Bases716.8 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA140828.32428082880708No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT100620.23737678535506No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT74014.88634077650372No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA3066.155703077851539No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA1322.655401327700664No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGAATCCTGTAGAACCAT290.5833836250251458No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC260.5230335948501308No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC210.4224502112251056No Hit
ACGTTGCCTTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGG170.3419835043250855No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC170.3419835043250855No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC170.3419835043250855No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC120.24140012070006037No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT110.2212834439750553No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC110.2212834439750553No Hit
ACGTTGCCTTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACC100.20116676725005028No Hit
GCATCAATCATACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG100.20116676725005028No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGAATCCTGTAGAAC90.18105009052504525No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC90.18105009052504525No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGCCCCTCTT90.18105009052504525No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGAATCCTGTAGAAC90.18105009052504525No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG90.18105009052504525No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC90.18105009052504525No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCACTCTT80.16093341380004023No Hit
GCATCAATCTCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG80.16093341380004023No Hit
GCATCAATCATCACAGGGTTGGCCAGTCACTAATGGGAATCCTGTAGAAC80.16093341380004023No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGAATCCTGTAGAAC80.16093341380004023No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGAATCCTGTAGAAC80.16093341380004023No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGGTGTCCCTCTT70.1408167370750352No Hit
GCATCAATCATCACAGGGTTGGCCAGCTAACTAATGGGAATCCTGTAGAA70.1408167370750352No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGAATCCTGTAGAAC70.1408167370750352No Hit
CATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.1408167370750352No Hit
ACGTTGCCTTTATCCCCCCGACGTACATGATCTGAATCCTGTAGAACCAT70.1408167370750352No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.1408167370750352No Hit
GCATCAATATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG70.1408167370750352No Hit
GCATCAATCACACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.1408167370750352No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.12070006035003018No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGAATCCTGTAGAAC60.12070006035003018No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGAATCCTGTAGAA60.12070006035003018No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCCCTT60.12070006035003018No Hit
ACGTTGCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCAT60.12070006035003018No Hit
GCATCAATCATCACAGGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGA60.12070006035003018No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.12070006035003018No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.12070006035003018No Hit
GATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG60.12070006035003018No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.12070006035003018No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCCTAATGGGAATCCTGTAGAAC60.12070006035003018No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG50.10058338362502514No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.10058338362502514No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGCTGTCCCTCTT50.10058338362502514No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC50.10058338362502514No Hit
GCATCAATCATCACGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG50.10058338362502514No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.10058338362502514No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.10058338362502514No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCCT50.10058338362502514No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCTCTCTT50.10058338362502514No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT50.10058338362502514No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)