FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3532
Total Bases509.4 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT146741.53454133635334No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA87024.631936579841447No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA802.2650056625141564No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA661.868629671574179No Hit
ACGTTGCCTTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGG290.8210645526613816No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT270.7644394110985278No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC190.5379388448471121No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGAATCCTGTAGAACCAT170.4813137032842582No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT120.33975084937712347No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG120.33975084937712347No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT110.3114382785956965No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC100.28312570781426954No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG100.28312570781426954No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC100.28312570781426954No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG90.2548131370328426No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGAATCCTGTAGAAC80.22650056625141565No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC80.22650056625141565No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT80.22650056625141565No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG80.22650056625141565No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.19818799546998866No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAGCTGGT70.19818799546998866No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.19818799546998866No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGAATCCTGTAGAAC70.19818799546998866No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT70.19818799546998866No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC70.19818799546998866No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGAATCCTGTAGAA70.19818799546998866No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.16987542468856173No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.16987542468856173No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGAATCCTGTAGAAC60.16987542468856173No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.16987542468856173No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.16987542468856173No Hit
ACGTTGCCTTAATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.16987542468856173No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.16987542468856173No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.16987542468856173No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGACCTGGT60.16987542468856173No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.14156285390713477No Hit
ACGTTGCCTTTAACCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT50.14156285390713477No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGA50.14156285390713477No Hit
ACGTTGCCTTTATCCTCCCAGACCCAGGAGTGGGGCCCATTCGATCTGGT50.14156285390713477No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGTGCCCATTCGATCTGGT40.11325028312570783No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.11325028312570783No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGCGGGGCCCATTCGATCTGGT40.11325028312570783No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTTGATCTGGT40.11325028312570783No Hit
GCATCAATCATCACAGGGTTGGCCGGCTCACTAATGGGAATCCTGTAGAA40.11325028312570783No Hit
CATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.11325028312570783No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT40.11325028312570783No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.11325028312570783No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATTTGGT40.11325028312570783No Hit
ACGTTCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG40.11325028312570783No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATATGGT40.11325028312570783No Hit
GCATCAATCACACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.11325028312570783No Hit
ACGATGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.11325028312570783No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)