FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3974
Total Bases573 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT141235.53095118268747No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA103926.144942123804732No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCACGAGATGTCCCTCTT1343.3719174635128337No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT1172.9441368897835933No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA942.3653749370910924No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA902.264720684448918No Hit
ACGTTGCCTTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGG290.7297433316557624No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT200.5032712632108707No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC160.40261701056869653No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC140.35228988424760943No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG140.35228988424760943No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT130.32712632108706596No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC130.32712632108706596No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGAATCCTGTAGAACCAT100.25163563160543534No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC100.25163563160543534No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGAATCCTGTAGAAC90.22647206844489182No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC90.22647206844489182No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGAATCCTGTAGAAC90.22647206844489182No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT80.20130850528434827No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGCTCTGGT80.20130850528434827No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGAATCCTGTAGAAC70.17614494212380472No Hit
GCATCAATCATCACGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.17614494212380472No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC70.17614494212380472No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.17614494212380472No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT70.17614494212380472No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.17614494212380472No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.1509813789632612No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.1509813789632612No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGAATCCTGTAGAAC60.1509813789632612No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGAATCCTGTAGAAC60.1509813789632612No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.1509813789632612No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCCTAATGGGAATCCTGTAGAAC60.1509813789632612No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.1509813789632612No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG60.1509813789632612No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCCGGT50.12581781580271767No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.12581781580271767No Hit
ACGTTGCCTTTATCCTCCCGGATCCAGGAGTGGGGCCCATTCGATCTGGT50.12581781580271767No Hit
ACGTTGCCTTTATCCCCCCGACGTACATGATCTGAATCCTGTAGAACCAT50.12581781580271767No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGAGCCCATTCGATCTGGT50.12581781580271767No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGAATCCTGTAGAA50.12581781580271767No Hit
GCATCAATCATCACAGGGTTGGCCAGTCACTAATGGGAATCCTGTAGAAC50.12581781580271767No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.12581781580271767No Hit
GCATCAATAATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA50.12581781580271767No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAC50.12581781580271767No Hit
GCATCAATCATCACAGGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGA50.12581781580271767No Hit
ACGTTGCCTTTTCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.12581781580271767No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCAGGT50.12581781580271767No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCGTTCGATCTGGT40.10065425264217413No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAGCTGGT40.10065425264217413No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG40.10065425264217413No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.10065425264217413No Hit
ACGTTGCCTTTATCCTCCCATATCCAGGAGTGGGGCCCATTCGATCTGGT40.10065425264217413No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGGGTGGGGCCCATTCGATCTGGT40.10065425264217413No Hit
ACGTTGCCTATATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.10065425264217413No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT40.10065425264217413No Hit
ACGTTGCCTTTATCCTCGACGTACATGATCTGAATCCTGTAGAACCATGT40.10065425264217413No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGC40.10065425264217413No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG40.10065425264217413No Hit
ACGTAGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT40.10065425264217413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)