FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2958
Total Bases426.9 kbp
Sequences flagged as poor quality0
Sequence length143-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT103835.09127789046653No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA88429.88505747126437No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA963.2454361054766734No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA612.0622041920216363No Hit
ACGTTGCCTTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGG331.1156186612576064No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT180.6085192697768762No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG180.6085192697768762No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT160.5409060175794456No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC120.4056795131845842No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC120.4056795131845842No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC110.37187288708586885No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC100.3380662609871535No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC90.3042596348884381No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGAATCCTGTAGAAC70.23664638269100743No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCCTAATGGGAATCCTGTAGAAC70.23664638269100743No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGAATCCTGTAGAAC60.2028397565922921No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.2028397565922921No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGAACTGGT60.2028397565922921No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGAATCCTGTAGAAC60.2028397565922921No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGAATCCTGTAGAAC60.2028397565922921No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.2028397565922921No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.2028397565922921No Hit
ACGATGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.2028397565922921No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGAATCCTGTAGAAC60.2028397565922921No Hit
ACGTTGCCTTTATCCTCCCACAGAGCCTTGGCATGCTCATAGAATTCGGG50.16903313049357674No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGACCATTCGATCTGGT50.16903313049357674No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGC50.16903313049357674No Hit
ACGTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.16903313049357674No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.16903313049357674No Hit
ACGTTGCCTTTATCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.16903313049357674No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.16903313049357674No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.1352265043948614No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGGGTGGGGCCCATTCGATCTGGT40.1352265043948614No Hit
GCATCAATCATCACAGGGTTGGCCGCTCACTAATGGGAATCCTGTAGAAC40.1352265043948614No Hit
ACGTTGCCTTTATCCTCCCAGATCCGGGAGTGGGGCCCATTCGATCTGGT40.1352265043948614No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.1352265043948614No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCAGTCGATCTGGT40.1352265043948614No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGCCCATTCGATCTGGTG40.1352265043948614No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCCTTCGATCTGGT40.1352265043948614No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCGATTCGATCTGGT40.1352265043948614No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGAGCCCATTCGATCTGGT40.1352265043948614No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTAGATCTGGT40.1352265043948614No Hit
GCACAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.1352265043948614No Hit
ACGTTGCCTTTATCCTCCCAACGTGAGGCACCAACATGCCACGTTTGGCC40.1352265043948614No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.10141987829614604No Hit
ACATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA30.10141987829614604No Hit
ACTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG30.10141987829614604No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTTTAGAA30.10141987829614604No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG30.10141987829614604No Hit
ACGTTGCCTTTATCCTCCCAGATCAAGGAGTGGGGCCCATTCGATCTGGT30.10141987829614604No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGAGGCCCATTCGATCTGGT30.10141987829614604No Hit
ACGTTCCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT30.10141987829614604No Hit
ACGTTGACTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT30.10141987829614604No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGAT30.10141987829614604No Hit
ACGTTGCCTTTACCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT30.10141987829614604No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGAATCCTGTAGAAC30.10141987829614604No Hit
ACGTTGCCTTTATCCTCCCAGATCCTGGAGTGGGGCCCATTCGATCTGGT30.10141987829614604No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC30.10141987829614604No Hit
GCATAAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA30.10141987829614604No Hit
AGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG30.10141987829614604No Hit
ACGTTGCCTTTATCCTCGACGTACATGATCTGAATCCTGTAGAACCATGT30.10141987829614604No Hit
ACGTTGCCTTTATCCCCGACGTACATGATCTGAATCCTGTAGAACCATGT30.10141987829614604No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGG30.10141987829614604No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGAATCCTGTAGAACCAT30.10141987829614604No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCGCATTCGATCTGGT30.10141987829614604No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGAATCCTGTAGAA30.10141987829614604No Hit
GCATCAATCATCACAGGGTTGGCCAGTCACTAATGGGAATCCTGTAGAAC30.10141987829614604No Hit
GCATCAATAATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA30.10141987829614604No Hit
GCATCAATCATCACAGGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGA30.10141987829614604No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)