FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4230
Total Bases609.2 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA132531.32387706855792No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT89121.06382978723404No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT58513.829787234042554No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA1363.2151300236406617No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA1293.0496453900709217No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGAATCCTGTAGAACCAT230.5437352245862884No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC220.5200945626477541No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC200.4728132387706856No Hit
ACGTTGCCTTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGG200.4728132387706856No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC140.3309692671394799No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT130.3073286052009456No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC130.3073286052009456No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC120.28368794326241137No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGAATCCTGTAGAAC120.28368794326241137No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGAATCCTGTAGAAC120.28368794326241137No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC120.28368794326241137No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC100.2364066193853428No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC100.2364066193853428No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCCTAATGGGAATCCTGTAGAAC100.2364066193853428No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG90.2127659574468085No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC90.2127659574468085No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGAATCCTGTAGAAC80.18912529550827423No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC80.18912529550827423No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGAATCCTGTAGAAC70.16548463356973994No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.16548463356973994No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGCTGTCCCTCTT70.16548463356973994No Hit
CATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.16548463356973994No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGAATCCTGTAGAA70.16548463356973994No Hit
ACGTTGCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG70.16548463356973994No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC70.16548463356973994No Hit
GCATCAATCATCACAGGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGA70.16548463356973994No Hit
ACGTTGCCGTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT60.14184397163120568No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.14184397163120568No Hit
GCATCAATCATCCAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG60.14184397163120568No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGGTGTCCCTCTT60.14184397163120568No Hit
GCATCAATCATCACCGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT60.14184397163120568No Hit
ACGTTGCCTTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACC60.14184397163120568No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.1182033096926714No Hit
CGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.1182033096926714No Hit
GCATCAATCATCACGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC50.1182033096926714No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGCCCCTCTT50.1182033096926714No Hit
CATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG50.1182033096926714No Hit
GCATCAATCATCAAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG50.1182033096926714No Hit
ACGTTGCCTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGTG50.1182033096926714No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)