FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008131371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008131371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1602
Total Bases231.4 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA74546.504369538077405No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTT23114.41947565543071No Hit
ACGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT794.931335830212235No Hit
GCATCAATCATCACAGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA744.619225967540574No Hit
ACGTTGCCTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACCA543.3707865168539324No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGAATCCTGTAGAAC130.8114856429463172No Hit
GCATCAATCATCACAGGGTTGCCAGCTCACTAATGGGAATCCTGTAGAAC110.686641697877653No Hit
ACGTTGCCTTTATCCTCCCGACGTACATGATCTGAATCCTGTAGAACCAT90.5617977528089888No Hit
CATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC80.4993757802746567No Hit
GCATCAATATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC80.4993757802746567No Hit
GCATCAATCATCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC80.4993757802746567No Hit
GCATAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC60.37453183520599254No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAAGGGAATCCTGTAGAAC60.37453183520599254No Hit
GCATCAATCATCACAGGGTTGGCCACTCACTAATGGGAATCCTGTAGAAC50.3121098626716604No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGAAGAA50.3121098626716604No Hit
GCATCAATCATCACAGGGTTGGCCAGTCACTAATGGGAATCCTGTAGAAC50.3121098626716604No Hit
GCATCATCATCACAGGGTTGGACTCGAGAAATCGCGAGATGTCCCTCTTG50.3121098626716604No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATGGGAATCCTGTAGAAC40.24968789013732834No Hit
GCTCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.24968789013732834No Hit
GCATCAATCATCAAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC40.24968789013732834No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTATTGGGAATCCTGTAGAA30.18726591760299627No Hit
GCATCAATCATCACAGGGTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.18726591760299627No Hit
ACGTTGCCTTTATCCTCCCCCGACGTACATGATCTGAATCCTGTAGAACC30.18726591760299627No Hit
GCATCAATCATCACAGGGTTTGCCAGCTCACTAATGGGAATCCTGTAGAA30.18726591760299627No Hit
GCATCAATCATCCCAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAA30.18726591760299627No Hit
GCATCAATCATACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.18726591760299627No Hit
GCATCAATCATCACAGGGTTGGACTCGAGGAATCGCGAGATGTCCCTCTT30.18726591760299627No Hit
GCACAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.18726591760299627No Hit
GCATCAATCATCACAGGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGA30.18726591760299627No Hit
GCATCAATCATCACAGGGTTGACCAGCTCACTAATGGGAATCCTGTAGAA30.18726591760299627No Hit
GCATCAATCATCACAGGGTTGGCCAGCTAACTAATGGGAATCCTGTAGAA30.18726591760299627No Hit
GGCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGA30.18726591760299627No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCCTAATGGGAATCCTGTAGAAC30.18726591760299627No Hit
GCATCAATCATCACAGGGTTGGCCAGCTACTAATGGGAATCCTGTAGAAC30.18726591760299627No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCATAATGGGAATCCTGTAGAAC30.18726591760299627No Hit
GATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC30.18726591760299627No Hit
GCATCAATCATCACAGGTTTGGCCACTCACTAATGGGAATCCTGTAGAAC20.12484394506866417No Hit
GCATCAATCATCACAGGGTTGGCCAGCCACTAATGGGAATCCTGTAGAAC20.12484394506866417No Hit
GCATCAACATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.12484394506866417No Hit
ACGTTGCCTTTTATCCTCCCCGACGTACATGATCTGAATCCTGTAGAACC20.12484394506866417No Hit
GCATCAATCATCACAGGGTTGGCCAGCACACTAATGGGAATCCTGTAGAA20.12484394506866417No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGCGAATCCTGTAGAA20.12484394506866417No Hit
GCATCAATCATCACAGGGTTGGCAGCTCACTAATGGGAATCCTGTAGAAC20.12484394506866417No Hit
GCATCAATCATCACAGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.12484394506866417No Hit
ACGTTGCCTTTATCCCCGACGTACATGATCTGAATCCTGTAGAACCATGT20.12484394506866417No Hit
GCATCATCATCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.12484394506866417No Hit
GCATCAATCATCACGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.12484394506866417No Hit
GCATCAATCATCACAGGTTTGGCCAGTCACTAATGGGAATCCTGTAGAAC20.12484394506866417No Hit
GCATCAATCATCACAGGGTTGGGCTCGAGAAATCGCGAGATGTCCCTCTT20.12484394506866417No Hit
GCATCAATCTCACAGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.12484394506866417No Hit
GCATCAATCATCACAGGGTTGGCCGCTCACTAATGGGAATCCTGTAGAAC20.12484394506866417No Hit
CCGTTGCCTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGGT20.12484394506866417No Hit
ACGTTGCCTTTATCATCCCCGACGTACATGATCTGAATCCTGTAGAACCA20.12484394506866417No Hit
GCATCAATCATCACGGGTTTGGCCAGCTCACTAATGGGAATCCTGTAGAA20.12484394506866417No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCCACTAATGGGAATCCTGTAGA20.12484394506866417No Hit
GCATCAATCATCATGGGTTGGCCAGCTCACTAATGGGAATCCTGTAGAAC20.12484394506866417No Hit
GCATCAATCATCACAGGGTTGGACTCGAGAAATCGCGAGAAGTCCCTCTT20.12484394506866417No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACTAATGGGACTCCTGTAGAA20.12484394506866417No Hit
GCATCAATCATCACAGGGTTGGCCAGCTCACAATGGGAATCCTGTAGAAC20.12484394506866417No Hit
ACGTTGCCTTTTATCCTCCCAGATCCAGGAGTGGGGCCCATTCGATCTGG20.12484394506866417No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)